ZNF667 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-77357

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human MIPU1. The immunogen is located within the first 50 amino acids of MIPU1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF667 Antibody - BSA Free

Western Blot: ZNF667 AntibodyBSA Free [NBP1-77357]

Western Blot: ZNF667 AntibodyBSA Free [NBP1-77357]

Western Blot: ZNF667 Antibody [NBP1-77357] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.
ZNF667 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZNF667 Antibody - BSA Free [NBP1-77357] -

Immunocytochemistry/ Immunofluorescence: ZNF667 Antibody - BSA Free [NBP1-77357] - Immunofluorescence of MIPU in human brain tissue with MIPU antibody at 20 u/mL.
ZNF667 Antibody - BSA Free

Immunohistochemistry: ZNF667 Antibody - BSA Free [NBP1-77357] -

Immunohistochemistry: ZNF667 Antibody - BSA Free [NBP1-77357] - Immunohistochemistry of ZNF667 in human brain tissue with ZNF667 antibody at 2.5 ug/mL.
ZNF667 Antibody - BSA Free

Western Blot: ZNF667 Antibody - BSA Free [NBP1-77357] -

ZNF667 and ZNF667-AS1 as novel markers for prognosis in PDAC.a Immunohistochemistry of ZNF667 shows variations of abundance across patients using the tissue microarray analysis. b Overall survival and progression-free survival of self-collected PDAC patients grouped by the abundance of ZNF667. c Relative abundance of ZNF667 and ZNF667-AS1 to ACTB mRNA in normal pancreas cells (HPDE6-C7), human embryonic kidney cells (HEK293T), liver metastasis pancreas cancer cells (L3.7), and the pancreas cancer cells detected by RT-qPCR. All pancreas cancer cells showed a decreased level of ZNF667 and ZNF667-AS1. *P < 0.05; **P < 0.01. d Immunoblot showing the decreased abundance of ZNF667 in multiple pancreas cancer cell lines. The abundance was calculated at the bottom. e, f Validation of ZNF667 and ZNF667-AS1 overexpression (OE) in two pancreas cancer cell lines. **P < 0.01; ****P < 0.0001. g Immunoblot showing the abundance of ZNF667 in PANC-1 and SW1990 cells before and after overexpression. h, i Proliferation evaluation (CCK8-positive cell ratio) after ZNF667 and ZNF667-AS1 overexpression in two pancreas cancer cell lines. n = 3 for each time point. **P < 0.001; ****P < 0.00001. j Evaluation of cellular apoptosis after ZNF667-AS1 overexpression in two pancreas cancer cell lines. n.s., no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35165277), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ZNF667 Antibody - BSA Free

Western Blot: ZNF667 Antibody - BSA Free [NBP1-77357] -

ZNF667 and ZNF667-AS1 as novel markers for prognosis in PDAC.a Immunohistochemistry of ZNF667 shows variations of abundance across patients using the tissue microarray analysis. b Overall survival and progression-free survival of self-collected PDAC patients grouped by the abundance of ZNF667. c Relative abundance of ZNF667 and ZNF667-AS1 to ACTB mRNA in normal pancreas cells (HPDE6-C7), human embryonic kidney cells (HEK293T), liver metastasis pancreas cancer cells (L3.7), and the pancreas cancer cells detected by RT-qPCR. All pancreas cancer cells showed a decreased level of ZNF667 and ZNF667-AS1. *P < 0.05; **P < 0.01. d Immunoblot showing the decreased abundance of ZNF667 in multiple pancreas cancer cell lines. The abundance was calculated at the bottom. e, f Validation of ZNF667 and ZNF667-AS1 overexpression (OE) in two pancreas cancer cell lines. **P < 0.01; ****P < 0.0001. g Immunoblot showing the abundance of ZNF667 in PANC-1 and SW1990 cells before and after overexpression. h, i Proliferation evaluation (CCK8-positive cell ratio) after ZNF667 and ZNF667-AS1 overexpression in two pancreas cancer cell lines. n = 3 for each time point. **P < 0.001; ****P < 0.00001. j Evaluation of cellular apoptosis after ZNF667-AS1 overexpression in two pancreas cancer cell lines. n.s., no significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35165277), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ZNF667 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF667

Mipu1, also known as zinc finger protein 667 or ZNF667, encodes a nuclear-localized protein containing 14 carboxy-terminal zinc finger motifs and an amino-terminal KRAB domain. This protein is highly expressed in heart and brain and is upregulated in rat heart after a transient ischemia-reperfusion procedure. Overexpression experiments suggest that Mipu1 suppresses the transcriptional activities of AP-1 and SRE in the MAPK signaling pathway and thus may play a role in the pathogenesis of cardiac and vascular disease. At least four isoforms of MIPU1 are known to exist.

Alternate Names

DKFZp686O111, FLJ14011, FLJ45518, MIPU1, myocardial ischemic preconditioning upregulated 1 ortholog, zinc finger protein 667

Gene Symbol

ZNF667

Additional ZNF667 Products

Product Documents for ZNF667 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ZNF667 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ZNF667 Antibody - BSA Free

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Protocols

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