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EpCAM/TROP-1 in the HT-29 Human Cell Line. Epithelial Cellular Adhesion Molecule (EpCAM/TROP1) was detected in immersion-fixed HT-29 human colon adenocarcinoma cells using a Mouse Anti-Human EpCAM/TROP1 Monoclonal Antibody (Catalog # MAB960). The cells were stained using the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Secondary Antibody (Catalog # NL009; green) and the nuclei were counterstained with DAPI (blue). Click here for a complete listing of malignancy-specific cancer stem cell markers.
Analysis of CD24low/-CD44+ Cells Isolated using the MagCellect™ Human CD24-CD44+ Breast Cancer Stem Cell Isolation Kit. A population of CD24low/-CD44+ cells was isolated from the MCF-7 human breast adenocarcinoma cell line using the MagCellect CD24low/-CD44+ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111). CD24low/-CD44+ (upper left quadrant), before (A) and after (B) enrichment, were detected by double-staining with APC-conjugated Mouse Anti-Human CD24 and PE-conjugated Mouse Anti-Human CD44 Detection Antibodies (both provided in the kit). A histogram profiling the enrichment of CD24low/- cells (filled histogram) from the original cell population (open histogram) is also shown (C).
Tumorsphere Formation using the StemXVivo™ Serum-Free Tumorsphere Media. The MCF-7 human breast cancer cell line was cultured for 7 days in StemXVivo Serum-Free Tumorsphere Media (Catalog # CCM012) to induce tumorsphere formation. The morphology of MCF-7 tumorspheres was documented using a CloneSelect™ Imager from Molecular Devices (New Milton).