Human EpCAM/TROP-1 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human EpCAM in direct ELISAs and Western blots. This antibody detects an epitope found in the extracellular domain between amino acids 136 and 265. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhMCAM,
    rhNCAM-L1, or rmOCAM is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 158210
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EpCAM
    Gln24-Lys265
    Accession # P16422
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human EpCAM/TROP‑1 Fc Chimera (Catalog # 960-EP)
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
EpCAM/TROP‑1 in HT-29 Human Cell Line. EpCAM/TROP‑1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
EpCAM/TROP‑1 in HT-29 Human Cell Line. EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
EpCAM/TROP‑1 in Human Adenocarcinoma. EpCAM/TROP‑1 was detected in immersion fixed paraffin-embedded sections of human adenocarcinoma using Mouse Anti-Human EpCAM/TROP‑1 Monoclonal Antibody (Catalog # MAB960) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with Trop-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

  • References:
    1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
    2. Boer, C.J. et al. (1999) J. Pathol. 188:201.
    3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
    4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
    5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
  • Long Name:
    Epithelial Cell Adhesion Molecule
  • Entrez Gene IDs:
    4072 (Human); 17075 (Mouse)
  • Alternate Names:
    17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis
    Authors: MJ Mitchell, J Webster, A Chung, PP Guimarães, OF Khan, R Langer
    Nat Commun, 2017;8(0):14179.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. Identification of tumorigenic cells and therapeutic targets in pancreatic neuroendocrine tumors
    Authors: GW Krampitz, BM George, SB Willingham, JP Volkmer, K Weiskopf, N Jahchan, AM Newman, D Sahoo, AJ Zemek, RL Yanovsky, JK Nguyen, PJ Schnorr, PK Mazur, J Sage, TA Longacre, BC Visser, GA Poultsides, JA Norton, IL Weissman
    Proc Natl Acad Sci USA, 2016;113(16):4464-9.
    Species: Human
    Sample Type:
  3. EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells that Promote Epithelial-to-Mesenchymal Transition
    Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell Surface Adhesion
  4. Integrative genomics identifies YY1AP1 as an oncogenic driver in EpCAM(+) AFP(+) hepatocellular carcinoma.
    Authors: Zhao X, Parpart S, Takai A, Roessler S, Budhu A, Yu Z, Blank M, Zhang Y, Jia H, Ye Q, Qin L, Tang Z, Thorgeirsson S, Wang X
    Oncogene, 2015;34(39):5095-104.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Detection of brain tumor cells in the peripheral blood by a telomerase promoter-based assay.
    Authors: Macarthur K, Kao G, Chandrasekaran S, Alonso-Basanta M, Chapman C, Lustig R, Wileyto E, Hahn S, Dorsey J
    Cancer Res, 2014;74(8):2152-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Activation of hepatic stem cell marker EpCAM by Wnt-beta-catenin signaling in hepatocellular carcinoma.
    Authors: Yamashita T, Budhu A, Forgues M, Wang XW
    Cancer Res., 2007;67(22):10831-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
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