Universal Sulfotransferase Activity Kit

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The Universal Sulfotransferase Activity Kit (Catalog # EA003) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sulfotransferases that use 3-phosphoadenosine-5-phosphosulfate (PAPS) as the donor substrate. This kit utilizes inositol monophosphatase 3 (IMPAD1) as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide 3-phosphoadenosine-5-phosphate (PAP) that is generated during sulfotransferase reactions.


  • Applicable for all sulfotransferases that use PAPS as the donor substrate
  • Non-radioactive
  • No time-consuming separation steps (e.g. column chromatography)
  • Provides accurate kinetic analysis of sulfotransferase reactions
  • Amenable to high-throughput analysis

Kit Contents

  • CHO cell-expressed Recombinant Mouse IMPAD1
  • PAP
  • Phosphatase Buffer 3
  • Phosphate Standard
  • Malachite Green Reagents A & B

Data Example

Activity of CHST7

Activity of CHST7
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The ability of Recombinant Mouse Carbohydrate Sulfotransferase 7/CHST7 (Catalog # 5108-ST) to transfer sulfate from PAPS to N-acetyl glucosamine was measured using the Universal Sulfotransferase Activity Kit (Catalog # EA003). The corrected optical densities were plotted versus the amount of enzyme. Specific activity of CHST7 was calculated to be 116.4 pmol/min/μg.