14-3-3 epsilon Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33554

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 14-3-3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 epsilon. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chimpanzee (100%).

Localization

Cytoplasm, Melanosome

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 epsilon Antibody - BSA Free

Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]

Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]

Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554]

Immunocytochemistry/ Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554]

Immunocytochemistry/Immunofluorescence: 14-3-3 epsilon Antibody [NBP1-33554] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554]

Immunohistochemistry-Paraffin: 14-3-3 epsilon Antibody [NBP1-33554] - Paraffin-embedded MDA-MB231 xenograft, using antibody at 1:500 dilution.
Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]

Western Blot: 14-3-3 epsilon Antibody [NBP1-33554]

Western Blot: 14-3-3 epsilon Antibody [NBP1-33554] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.

Applications for 14-3-3 epsilon Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: 14-3-3 epsilon

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Alternate Names

1433 epsilon, YWHAE

Gene Symbol

YWHAE

Additional 14-3-3 epsilon Products

Product Documents for 14-3-3 epsilon Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 14-3-3 epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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