14-3-3 gamma Antibody (AT4B9) - BSA Free

Novus Biologicals | Catalog # NBP2-50579

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # AT4B9

Format

BSA Free
View all 14-3-3 gamma Primary Antibodies »

Product Specifications

Immunogen

Recombinant human 14-3-3 gamma (1-247aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for 14-3-3 gamma Antibody (AT4B9) - BSA Free

Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate
Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) - BSA Free [NBP2-50579]

Immunocytochemistry/ Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) - BSA Free [NBP2-50579]

Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9)BSA Free [NBP2-50579]

Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.

Applications for 14-3-3 gamma Antibody (AT4B9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 gamma

14-3-3 protein gamma is a member of the 14-3-3 family of proteins which help mediate signal transduction. Specifically, 14-3-3 gamma is an adapter protein responsible for bringing signal transduction to completion. It therefore plays an important role in regulating many fundamental cellular processes, such as apoptosis and cell-cycle checkpoints.

Signal-induced phosphorylation has the ability to change protein function, however phosphorylation is not always enough to change a protein's function. 14-3-3 gamma has the ability to bind a large number of target proteins thereby affecting the proteins' activity and/or function.

14-3-3 gamma is highly expressed in brain, heart and skeletal muscle, where it is induced by growth factors in human vascular smooth muscle cells. This suggests that 14-3-3 gamma may play a role in muscle tissue function.

Alternate Names

1433 gamma, YWHAG

Gene Symbol

YWHAG

Additional 14-3-3 gamma Products

Product Documents for 14-3-3 gamma Antibody (AT4B9) - BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 gamma Antibody (AT4B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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