14-3-3 tau/theta Antibody (1A1) - BSA Free

Novus Biologicals | Catalog # NBP1-21301

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A1

Format

BSA Free
View all 14-3-3 theta Primary Antibodies »

Product Specifications

Immunogen

Recombinant human 14-3-3 tau/theta (1-245aa) purified from E. coli (NP_006817).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 tau/theta Antibody (1A1) - BSA Free

Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MDA-MB-21 cell lysateLane 2. : HeLa cell lysateLane 3. : A431 cell lysateLane 4. : NIH3T3 cell lysateLane 5. : 293T cell lysateLane 6. : HepG2 cell lysate
Immunocytochemistry/ Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) - BSA Free [NBP1-21301]

Immunocytochemistry/ Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) - BSA Free [NBP1-21301]

Immunocytochemistry/Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - 14-3-3 tau/theta Antibody (1A1) Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 theta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1)BSA Free [NBP1-21301]

Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PDVF membrane and probed with antihuman 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human YWHAZLane 2. : Recombinant Human YWHABLane 3. : Recombinant Human YWHAELane 4. : Recombinant Human YWHAHLane 5. : Recombinant Human YWHAGLane 6. : Recombinant Human SFNLane 7. : Recombinant Human YWHAQ (14-3-3 tau/theta)

Applications for 14-3-3 tau/theta Antibody (1A1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-21301 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 theta

14-3-3 theta belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and it is 99% identical to the mouse and rat orthologs. 14-3-3 theta is upregulated in patients with amyotrophic lateral sclerosis (1).

Alternate Names

14-3-3 protein tau, 1433 theta, HS1 protein, YWHAQ

Entrez Gene IDs

10971 (Human)

Gene Symbol

YWHAQ

UniProt

P27348

Additional 14-3-3 theta Products

Product Documents for 14-3-3 tau/theta Antibody (1A1) - BSA Free

Certificate of Analysis

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Product Specific Notices for 14-3-3 tau/theta Antibody (1A1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 14-3-3 tau/theta Antibody (1A1) - BSA Free

Customer Reviews for 14-3-3 tau/theta Antibody (1A1) - BSA Free (1)

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  • 14-3-3 tau/theta Antibody (1A1)
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: 293T and MCF-7 cells-whole cell lysate and MDA MB 231 cells
    Species: Human
    Verified Customer | Posted 03/20/2021
    Western Blot: HEK293T, MDA-MB-231 and MCF-7 whole cell lysates were loaded with 30 ug/lane. 10% SDS-PAGE. Mouse Monoclonal 14-3-3 tau/theta Antibody (1A1) (NBP1-21301) was used for primary antibody: 1:1000, 4℃, overnight.
    14-3-3 tau/theta Antibody (1A1) - BSA Free NBP1-21301

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