Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 016 expressed in HEK293

Format

Azide and BSA Free
View all 15-PGDH/HPGD Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human 15-PGDH/HPGD (Accession#: NP_000851.2; Met1-Gln266).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for 15-PGDH/HPGD Antibody (016) - Azide and BSA Free

Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839]

Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839]

Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated, stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
15-PGDH/HPGD Antibody (016) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] -

Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89839 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for 15-PGDH/HPGD Antibody (016) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 15-PGDH/HPGD

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Gene Symbol

HPGD

Additional 15-PGDH/HPGD Products

Product Documents for 15-PGDH/HPGD Antibody (016) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 15-PGDH/HPGD Antibody (016) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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