Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C10

Format

Azide and BSA Free
View all 15-PGDH/HPGD Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free (NBP2-00490) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

Western Blot: 15-PGDH/HPGD Antibody (OTI2C10)Azide and BSA Free [NBP2-70933]

Western Blot: 15-PGDH/HPGD Antibody (OTI2C10)Azide and BSA Free [NBP2-70933]

Western Blot: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY 15-PGDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-15-PGDH.
Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Human bladder tissue using anti-15-PGDH mouse monoclonal antibody.
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-15-PGDH antibody, and then analyzed by flow cytometry.
Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Immunohistochemistry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Staining of Adenocarcinoma of Human endometrium tissue using anti-15-PGDH mouse monoclonal antibody.
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Hela cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933]

Flow Cytometry: 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free [NBP2-70933] - Analysis of Jurkat cells, using anti-15-PGDH antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 15-PGDH/HPGD

15-PGDH (15-hydroxyprostaglandin dehydrogenase (NAD+)) is responsible for prostaglandin inactivation and supports the regulation of functions that are controled by prostaglandin levels. Specifically, 15PGDH catalyzes the chemical reaction to convert NAD+ to NADH and H+.

15-PGDH is a novel tumor suppressor in the COX-2 pathway. 15-PGDH is found in many normal tissues and appears to be down-regulated in colorectal and lung carcinoma tissue.

Long Name

15-Hydroxyprostaglandin Dehydrogenase

Alternate Names

15PGDH, HPGD, PGDH1, SDR36C1

Gene Symbol

HPGD

Additional 15-PGDH/HPGD Products

Product Documents for 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 15-PGDH/HPGD Antibody (OTI2C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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