Chemical Name: 2-Aminoethoxydiphenylborane
Biological Activity2-APB is a functional and membrane permeable D-myo-inositol 1,4,5-trisphosphate (IP3) receptor antagonist (IC50 = 42 μM). Stimulates store-operated calcium (SOC) release at low concentrations (< 10 μM) and inhibits it at higher concentrations (up to 50 μM). Increases STIM-Orai channel conductance and limits ion selectivity. Modulator of TRP channels; blocks TRPC1, TRPC3, TRPC5, TRPC6, TRPV6, TRPM3, TRPM7, TRPM8 and TRPP2 and at higher concentrations stimulates TRPV1, TRPV2 and TRPV3. Also blocks specific gap channel subtypes.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
STIM and Orai: the long-awaited constituents of store-operated calcium entry.
Varnai et al.
Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB).
Bai et al.
2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release.
Maruyama et al.
Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltafe-dependent effect.
Xu et al.
Inhibition of the transient receptor potential cation channel TRPM2 by 2-aminoethoxydiphenyl borate (2-APB).
Togashi et al.
Citations for 2-APB
The citations listed below are publications that use Tocris products. Selected citations for 2-APB include:
44 Citations: Showing 1 - 10
TRPM4 Conductances in Thalamic Reticular Nucleus Neurons Generate Persistent Firing during Slow Oscillations
Authors: O'Malley Et al.
J Neurosci 2020;40:4813
Cell sensing and decision-making in confinement: The role of TRPM7 in a tug of war between hydraulic pressure and cross-sectional area.
Authors: Zhao Et al.
Sci Adv 2019;5:eaaw7243
Role of miRNA-181a-2-3p in cadmium-induced inflammatory responses of human bronchial epithelial cells.
Authors: Kim Et al.
J Thorac Dis 2019;11:3055
Neuronal Activity-Dependent Activation of Astroglial Calcineurin in Mouse Primary Hippocampal Cultures.
Authors: Lim Et al.
Int J Mol Sci 2018;19
Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma.
Authors: Florea Et al.
Gpr158 mediates osteocalcin's regulation of cognition.
Authors: Khrimian Et al.
J Exp Med 2017;214:2859
Connexin 32-mediated cell-cell communication is essential for hepatic differentiation from humanembryonic stem cells
Authors: Qin Et al.
Scientific Reports 2016;6:37388
Layer-specific potentiation of network GABAergic inhibition in the CA1 area of the hippocampus.
Authors: Colavita Et al.
Sci Rep 2016;6:28454
Activation of the neurokinin 3 receptor promotes filopodia growth and sprouting in rat embryonic hypothalamic cells.
Authors: Flynn Et al.
The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine.
Authors: Baron Et al.
Dev Neurobiol 2015;6:e1629
Pathophysiological significance of the two-pore domain K(+) channel K2P5.1 in splenic CD4(+)CD25(-) T cell subset from a chemically-induced murine inflammatory bowel disease model.
Authors: Nakakura Et al.
Cell Death Dis 2015;6:299
Stimulation-evoked Ca2+ signals in astrocytic processes at hippocampal CA3-CA1 synapses of adult mice are modulated by glutamate and ATP.
Authors: Tang Et al.
J Neurosci 2015;35:3016
Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling.
Authors: Dobson Et al.
Biomed Res Int 2015;10:e0125974
Dissecting out the complex Ca2+-mediated phenylephrine-induced contractions of mouse aortic segments.
Authors: Fransen Et al.
PLoS One 2015;10:e0121634
Both neurons and astrocytes exhibited tetrodotoxin-resistant metabotropic glutamate receptor-dependent spontaneous slow Ca2+ oscillations in striatum.
Authors: Tamura Et al.
PLoS One 2014;9:e85351
Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process.
Authors: Hutchins and Wray
J Biol Chem 2014;8:400
Potent functional uncoupling between STIM1 and Orai1 by dimeric 2-aminodiphenyl borinate analogs.
Authors: Hendron Et al.
Cell Calcium 2014;56:482
Cross-synaptic synchrony and transmission of signal and noise across the mouse retina.
Authors: Grimes Et al.
Activation of a TRP-like channel and intracellular Ca2+ dynamics during phospholipase-C-mediated cell death.
Authors: Gonçalves Et al.
J Cell Sci 2014;127:3817
Endoplasmic reticulum membrane reorganization is regulated by ionic homeostasis.
Authors: Varadarajan Et al.
PLoS One 2013;8:e56603
Antagonism of neuronal prostaglandin E(2) receptor subtype 1 mitigates amyloid β neurotoxicity in vitro.
Authors: Li Et al.
Biochem Pharmacol 2013;8:87
AMP-activated protein kinase as regulator of P2Y(6) receptor-induced Ins secretion in mouse pancreatic β-cells.
Authors: Balasubramanian Et al.
Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition.
Authors: Chang Et al.
Front Cell Neurosci 2013;34:1096
Calcium release-dependent actin flow in the leading process mediates axophilic migration.
Authors: Hutchins Et al.
J Pharmacol Exp Ther 2013;33:11361
In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium.
Authors: Aihara Et al.
PLoS One 2013;288:33585
SKF83959, an agonist of phosphatidylinositol-linked D(1)-like receptors, promotes ERK1/2 activation and cell migration in cultured rat astrocytes.
Authors: Huang Et al.
Korean J Physiol Pharmacol 2012;7:e49954
The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
Authors: Lee Et al.
DHA inhibits ER Ca2+ release and ER stress in astrocytes following in vitro ischemia.
Authors: Begum Et al.
J Neurochem 2012;120:622
Regulation of Substantia Nigra Pars Reticulata GABAergic Neuron Activity by H?O? via Flufenamic Acid-Sensitive Channels and K(ATP) Channels.
Authors: Lee Et al.
Front Syst Neurosci 2011;5:14
Calcium-sensing receptor modulates extracellular Ca(2+) entry via TRPC-encoded receptor-operated channels in human aortic smooth muscle cells.
Authors: Chow Et al.
PLoS One 2011;301:C461
The amiloride-sensitive epithelial Na+ channel PPK28 is essential for drosophila gustatory water reception.
Authors: Chen Et al.
J Neurosci 2010;30:6247
Non-cardiomyocytes influence the electrophysiological maturation of human embryonic stem cell-derived cardiomyocytes during differentiation.
Authors: Kim Et al.
Front Physiol 2010;19:783
Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell.
Authors: Choi Et al.
J Neuroimmune Pharmacol 2010;14:105
Mobilization of calcium from intracellular stores facilitates somatodendritic DA release.
Authors: Patel Et al.
J Neurosci 2009;29:6568
Adenosine-evoked hyperpolarization of retinal ganglion cells is mediated by G-protein-coupled inwardly rectifying K+ and small conductance Ca2+-activated K+ channel activation.
Authors: Clark Et al.
J Neurosci 2009;29:11237
P2Y receptors mediate Ca2+ signaling in duodenocytes and contribute to duodenal mucosal bicarbonate secretion.
Authors: Dong Et al.
Am J Physiol Gastrointest Liver Physiol 2009;296:G424
Estrogen attenuates glutamate-induced cell death by inhibiting Ca2+ influx through L-type voltage-gated Ca2+ channels.
Authors: Sribnick Et al.
Brain Res 2009;1276:159
Regulated release of BDNF by cortical oligodendrocytes is mediated through metabotropic glutamate receptors and the PLC pathway.
Authors: Bagayogo and Dreyfus
ASN Neuro 2009;1
Low concentrations of sphingosylphosphorylcholine enhance pulmonary artery vasoreactivity: the role of protein kinase C δ and Ca2+ entry.
Authors: Snetkov Et al.
Am J Physiol Cell Physiol 2008;51:239
Calcium release from presynaptic internal stores is required for ethanol to increase spontaneous γ-aminobutyric acid release onto cerebellum Purkinje neurons.
Authors: Kelm Et al.
Stem Cells Dev 2007;323:356
Contribution of both Ca2+ entry and Ca2+ sensitization to the alpha1-adrenergic vasoconstriction of rat penile small arteries.
Authors: Villalba Et al.
Am J Physiol Heart Circ Physiol 2007;292:H1157
Nimodipine selectively stimulates beta-amyloid 1-42 secretion by a mechanism independent of calcium influx blockage.
Authors: Facchinetti Et al.
Neurobiol Aging 2006;27:218
PI3K and PKC contribute to membrane depolarization mediated by alpha2-adrenoceptors in the canine isolated mesenteric vein.
BMC Physiol 2005;5:9
2-aminoethoxydiphenyl borate stimulates pulmonary C neurons via the activation of TRPV channels.
Authors: Gu Et al.
Am J Physiol Lung Cell Mol Physiol 2005;288:L932
No product specific FAQs exist for this product, however you mayView all Small Molecule FAQs
Reviews for 2-APB
Average Rating: 4 (Based on 1 Review)
Have you used 2-APB?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
We used 2-APB to determine whether IP3R involved in the related calcium signaling