Catalog Number: 1224
Chemical Name: 2-Aminoethoxydiphenylborane
Biological Activity
A functional and membrane permeable D-myo-inositol 1,4,5-trisphosphate (IP3) receptor antagonist (IC50 = 42 μM). Stimulates store-operated calcium (SOC) release at low concentrations (< 10 μM) and inhibits it at higher concentrations (up to 50 μM). Increases STIM-Orai channel conductance and limits ion selectivity. Modulator of TRP channels; blocks TRPC1, TRPC3, TRPC5, TRPC6, TRPV6, TRPM3, TRPM7, TRPM8 and TRPP2 and at higher concentrations stimulates TRPV1, TRPV2 and TRPV3. Also blocks specific gap channel subtypes.
Technical Data
  • M.Wt:
    225.1
  • Formula:
    C14H16BNO
  • Solubility:
    Soluble to 10 mM in ethanol with gentle warming and to 100 mM in DMSO with gentle warming
  • Purity:
    >98%
  • Storage:
    Store at RT
  • CAS No:
    524-95-8
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. STIM and Orai: the long-awaited constituents of store-operated calcium entry.
    Varnai et al.
    TiPS, 2009;30:118
  2. Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB).
    Bai et al.
    J.Pharmacol.Exp.Ther., 2006;319:1452
  3. 2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release.
    Maruyama et al.
    J.Biochem., 1997;122:498
  4. Block of TRPC5 channels by 2-aminoethoxydiphenyl borate: a differential, extracellular and voltafe-dependent effect.
    Xu et al.
    Br.J.Pharmacol., 2005;145:405
  5. Inhibition of the transient receptor potential cation channel TRPM2 by 2-aminoethoxydiphenyl borate (2-APB).
    Togashi et al.
    Br.J.Pharmacol., 2008;153:1324
Citations:

The citations listed below are publications that use Tocris products. Selected citations for 2-APB include:

27 Citations: Showing 1 - 10
Filter your results:

  1. Connexin 32-mediated cell-cell communication is essential for hepatic differentiation from humanembryonic stem cells
    Authors: Qin Et al.
    Scientific Reports 2016;6:37388
  2. Activation of the neurokinin 3 receptor promotes filopodia growth and sprouting in rat embryonic hypothalamic cells.
    Authors: Flynn Et al.
    Carcinogenesis 2015;75:44896
  3. The NLRP3 inflammasome is activated by nanoparticles through ATP, ADP and adenosine.
    Authors: Baron Et al.
    Dev Neurobiol 2015;6:e1629
  4. Pathophysiological significance of the two-pore domain K(+) channel K2P5.1 in splenic CD4(+)CD25(-) T cell subset from a chemically-induced murine inflammatory bowel disease model.
    Authors: Nakakura Et al.
    Cell Death Dis 2015;6:299
  5. Stimulation-evoked Ca2+ signals in astrocytic processes at hippocampal CA3-CA1 synapses of adult mice are modulated by glutamate and ATP.
    Authors: Tang Et al.
    J Neurosci 2015;35:3016
  6. Caffeine Modulates Vesicle Release and Recovery at Cerebellar Parallel Fibre Terminals, Independently of Calcium and Cyclic AMP Signalling.
    Authors: Dobson Et al.
    Biomed Res Int 2015;10:e0125974
  7. Dissecting out the complex Ca2+-mediated phenylephrine-induced contractions of mouse aortic segments.
    Authors: Fransen Et al.
    PLoS One 2015;10:e0121634
  8. Both neurons and astrocytes exhibited tetrodotoxin-resistant metabotropic glutamate receptor-dependent spontaneous slow Ca2+ oscillations in striatum.
    Authors: Tamura Et al.
    PLoS One 2014;9:e85351
  9. Capture of microtubule plus-ends at the actin cortex promotes axophilic neuronal migration by enhancing microtubule tension in the leading process.
    Authors: Hutchins and Wray
    J Biol Chem 2014;8:400
  10. Cross-synaptic synchrony and transmission of signal and noise across the mouse retina.
    Authors: Grimes Et al.
    Elife 2014;3:e03892
  11. Endoplasmic reticulum membrane reorganization is regulated by ionic homeostasis.
    Authors: Varadarajan Et al.
    PLoS One 2013;8:e56603
  12. Antagonism of neuronal prostaglandin E(2) receptor subtype 1 mitigates amyloid β neurotoxicity in vitro.
    Authors: Li Et al.
    Biochem Pharmacol 2013;8:87
  13. AMP-activated protein kinase as regulator of P2Y(6) receptor-induced insulin secretion in mouse pancreatic β-cells.
    Authors: Balasubramanian Et al.
    Hypertension 2013;85:991
  14. Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition.
    Authors: Chang Et al.
    Front Cell Neurosci 2013;34:1096
  15. Calcium release-dependent actin flow in the leading process mediates axophilic migration.
    Authors: Hutchins Et al.
    J Pharmacol Exp Ther 2013;33:11361
  16. In vivo epithelial wound repair requires mobilization of endogenous intracellular and extracellular calcium.
    Authors: Aihara Et al.
    PLoS One 2013;288:33585
  17. SKF83959, an agonist of phosphatidylinositol-linked D(1)-like receptors, promotes ERK1/2 activation and cell migration in cultured rat astrocytes.
    Authors: Huang Et al.
    Korean J Physiol Pharmacol 2012;7:e49954
  18. The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
    Authors: Lee Et al.
    Nature 2012;492:123
  19. DHA inhibits ER Ca2+ release and ER stress in astrocytes following in vitro ischemia.
    Authors: Begum Et al.
    J Neurochem 2012;120:622
  20. Calcium-sensing receptor modulates extracellular Ca(2+) entry via TRPC-encoded receptor-operated channels in human aortic smooth muscle cells.
    Authors: Chow Et al.
    PLoS One 2011;301:C461
  21. Non-cardiomyocytes influence the electrophysiological maturation of human embryonic stem cell-derived cardiomyocytes during differentiation.
    Authors: Kim Et al.
    Front Physiol 2010;19:783
  22. Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell.
    Authors: Choi Et al.
    J Neuroimmune Pharmacol 2010;14:105
  23. Adenosine-evoked hyperpolarization of retinal ganglion cells is mediated by G-protein-coupled inwardly rectifying K+ and small conductance Ca2+-activated K+ channel activation.
    Authors: Clark Et al.
    J Neurosci 2009;29:11237
  24. P2Y receptors mediate Ca2+ signaling in duodenocytes and contribute to duodenal mucosal bicarbonate secretion.
    Authors: Dong Et al.
    Am J Physiol Gastrointest Liver Physiol 2009;296:G424
  25. Estrogen attenuates glutamate-induced cell death by inhibiting Ca2+ influx through L-type voltage-gated Ca2+ channels.
    Authors: Sribnick Et al.
    Brain Res 2009;1276:159
  26. Low concentrations of sphingosylphosphorylcholine enhance pulmonary artery vasoreactivity: the role of protein kinase C δ and Ca2+ entry.
    Authors: Snetkov Et al.
    Am J Physiol Cell Physiol 2008;51:239
  27. Calcium release from presynaptic internal stores is required for ethanol to increase spontaneous γ-aminobutyric acid release onto cerebellum Purkinje neurons.
    Authors: Kelm Et al.
    Stem Cells Dev 2007;323:356
Expand to show all 27 Citations

Have you used 2-APB?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details

Note:

Contact Information

Calculators

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

R&D Systems Guarantee

or select another batch: 

Feedback

Customer Information