5-hmC Antibody (RM236)

Novus Biologicals | Catalog # NBP2-61469

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Vertebrate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Methylated DNA Immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM236 expressed in HEK293
View all 5-hmC Primary Antibodies »

Product Specifications

Immunogen

BSA-conjugated 5-hmC

Specificity

This antibody reacts to 5-hmC in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-hmC Antibody (RM236)

Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] -

Immunohistochemistry: 5-hmC Antibody (RM236) [NBP2-61469] - Immunohistochemical staining of formalin fixed and paraffin embedded Human brain tissue sections, using NBP2-61469

Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] -

Flow Cytometry: 5-hmC Antibody (RM236) [NBP2-61469] - Flow Cytometry analysis of 5-hmC expression in HEK293 cells using NBP2-61470. The cells were fixed with ice-cold MeOH, permeabilized with 0.5% Triton X-100, denatured with 2N HCl, then stained with NBP2-61469 (Blue) or with a negative control antibody (red)
5-hmC Antibody (RM236)

Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] -

Methylated DNA Immunoprecipitation: 5-hmC Antibody (RM236) [NBP2-61469] - hMeDIP was performed using Anti-5-hmC antibody (NBP2-61469) at a 10:1 DNA: Ab ratio. 1 ng of unmethylated, 5-Methylcytosine (5-mC) or 5-Hydroxymethylcytosine (5-hmC) DNA standard (897 bp) was spiked in 1ug of genomic DNA isolated from HeLa cells as the control. Realtime PCR was then performed to determine the capture of DNA standard as in % of input.
5-hmC Antibody (RM236)

ELISA: 5-hmC Antibody (RM236) [NBP2-61469] -

ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - Direct ELISA of Mouse brain genomic DNA using Anti-5-hmC antibody (NBP2-61469). The plate was directly coated with different concentrations of genomic DNA isolated from Mouse brain tissue. NBP2-61469 was used as the primary antibody, and a HRP conjugated Anti-Rabbit IgG as the secondary antibody.
5-hmC Antibody (RM236)

ELISA: 5-hmC Antibody (RM236) [NBP2-61469] -

ELISA: 5-hmC Antibody (RM236) [NBP2-61469] - ELISA of single stranded DNA using Anti-5-hmC antibody (NBP2-61469). The plate was coated with streptavidin and then biotinylated single stranded unmethylated DNA, 5-Methylcytosine (5-mC) DNA, and 5-Hydroxymethylcytosine (5-hmC) DNA. A serial dilution of NBP2-61469 was used as the primary antibody, and an alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
5-hmC Antibody (RM236)

Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] -

Dot Blot: 5-hmC Antibody (RM236) [NBP2-61469] - Dot blot of double stranded DNA using NBP2-61469. The membrane was pre-spotted with double stranded 5-Hydroxymethylcytosine (5-hmC) DNA, 5-Methylcytosine (5-mC) DNA, and unmethylated DNA.
5-hmC Antibody (RM236)

Immunocytochemistry/Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] -

Immunocytochemistry/Immunofluorescence: 5-hmC Antibody (RM236) [NBP2-61469] - Immunocytochemical staining of HeLa cells using NBP2-61469 at 0.5 ug/ml (red). Actin filaments was labeled with fluorescein phalloidin green). HeLa cells were fixed with 4% paraformaldehyde and permeabilized with methanol (-20C) before treatment with 2 N HCl for 30 min at 37C to denature the DNA.

Applications for 5-hmC Antibody (RM236)

Application
Recommended Usage

Dot Blot

0.2 ug/ml - 1 ug/ml

ELISA

0.1 ug/ml - 1 ug/ml

Flow Cytometry

1 ug/ml-5 ug/ml

Immunocytochemistry/ Immunofluorescence

0.5 ug/ml - 2 ug/ml

Immunohistochemistry

0.1 ug/ml - 1 ug/ml

Methylated DNA Immunoprecipitation

0.2 ug/ml - 2ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-hmC

Alternate Names

5'-hmC, 5-Hydroxymethylcytosine

Additional 5-hmC Products

Product Documents for 5-hmC Antibody (RM236)

Certificate of Analysis

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Product Specific Notices for 5-hmC Antibody (RM236)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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