5-HT2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-16043

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-HT2A Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 5-HT2A (NP_000612.1). MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT2A Antibody - BSA Free

Western Blot: 5-HT2A AntibodyAzide and BSA Free [NBP3-16043]

Western Blot: 5-HT2A AntibodyAzide and BSA Free [NBP3-16043]

Western Blot: 5-HT2A Antibody [NBP3-16043] - Analysis of extracts of various cell lines, using 5-HT2A antibody (NBP3-16043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
5-HT2A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody - Azide and BSA Free [5-HT2A] -

Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody - Azide and BSA Free [5-HT2A] - Immunofluorescence analysis of Neuro-2a cells using 5-HT2A Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for 5-HT2A Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-HT2A

The 5HT2A Receptor gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene areassociated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated withresponse to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also havea mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressantdownregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found forthis gene. (provided by RefSeq)

Long Name

5-Hydroxytryptamine Receptor 2A

Alternate Names

5HT2A, HTR2A

Gene Symbol

HTR2A

Additional 5-HT2A Products

Product Documents for 5-HT2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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