5-HT2C Antibody (9H8) - BSA Free

Novus Biologicals | Catalog # NBP3-26213

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H8 expressed in HEK293

Format

BSA Free
View all 5-HT2C Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human 5-HT2C [UniProt P28335]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-HT2C Antibody (9H8) - BSA Free

5-HT2C Antibody (9H8)

Immunohistochemistry: 5-HT2C Antibody (9H8) [NBP3-26213] -

Immunohistochemistry: 5-HT2C Antibody (9H8) [NBP3-26213] - Image of 5-HT2C Antibody (9H8) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
5-HT2C Antibody (9H8)

Western Blot: 5-HT2C Antibody (9H8) [NBP3-26213] -

Western Blot: 5-HT2C Antibody (9H8) [NBP3-26213] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.
All lanes: 5-HT2C Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 52, 29 kDa
Observed band size: 75 kDa

Applications for 5-HT2C Antibody (9H8) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: 5-HT2C

Serotonin (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract. Serotonin plays an important part in the biochemistry of depression, migraine, bipolar disorder and anxiety. It is also believed to be influential on sexuality and appetite. 5-HT is generally thought not to be released from synaptic terminal buttons in the manner of classical neurotransmission but from serotonergic varacosities into the extra neuronal space. From here it is free to diffuse over a relatively large region of space (>20um) and activate 5-HT receptors located on the dendrites, cell bodies and presynaptic terminals of adjacent neurons. Serotonergic action is terminated primarily via uptake of 5-HT from the synapse. This is through the specific monoamine transporter for 5-HT, 5-HT reuptake transporter, on the presynaptic neuron. The pharmacology of 5-HT is extremely complex, with its actions being mediated by a large and diverse range of 5-HT receptors.

Long Name

5-Hydroxytryptamine Receptor 2C

Alternate Names

5-HTR2C, 5HT2C, HTR2C, Serotonin Receptor 2C

Gene Symbol

HTR2C

Additional 5-HT2C Products

Product Documents for 5-HT2C Antibody (9H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-HT2C Antibody (9H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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