5-HT3B Antibody (2H9H5) - BSA Free

Novus Biologicals | Catalog # NBP2-61787

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2H9H5

Format

BSA Free
View all 5-HT3B Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human 5-HT3B (AA: extra 23-150) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

50.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5-HT3B Antibody (2H9H5) - BSA Free

Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mAb against HEK293 (1) and HTR3B (AA: extra 23-150)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: 5-HT3B Antibody (2H9H5) - BSA Free [NBP2-61787]

Immunocytochemistry/ Immunofluorescence: 5-HT3B Antibody (2H9H5) - BSA Free [NBP2-61787]

Immunocytochemistry/Immunofluorescence: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: 5-HT3B Antibody (2H9H5) - BSA Free [NBP2-61787]

Flow Cytometry: 5-HT3B Antibody (2H9H5) - BSA Free [NBP2-61787]

Flow Cytometry: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis of Hela cells using HTR3B mouse mAb (green) and negative control (red).
Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mAb against human HTR3B (AA: extra 23-150) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5)BSA Free [NBP2-61787]

Western Blot: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Analysis using HTR3B mouse mAb against SK-N-SH (1) and Hela (2) cell lysate.
ELISA: 5-HT3B Antibody (2H9H5) [NBP2-61787] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for 5-HT3B Antibody (2H9H5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT3B

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It is not functional as a homomeric complex, but a pentaheteromeric complex with subunit A (HTR3A) displays the full functional features of this receptor.

Long Name

5-Hydroxytryptamine Receptor 3B

Alternate Names

5HT3B, HTR3B

Gene Symbol

HTR3B

Additional 5-HT3B Products

Product Documents for 5-HT3B Antibody (2H9H5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5-HT3B Antibody (2H9H5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for 5-HT3B Antibody (2H9H5) - BSA Free

There are currently no reviews for this product. Be the first to review 5-HT3B Antibody (2H9H5) - BSA Free and earn rewards!

Have you used 5-HT3B Antibody (2H9H5) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies