5-Lipoxygenase [p Ser271] Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05439

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all 5-Lipoxygenase Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S271 of human 5-Lipoxygenase (NP_000689.1). QLSLE

Modification

p Ser271

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Western Blot: 5-Lipoxygenase [p Ser271] AntibodyBSA Free [NBP3-05439]

Western Blot: 5-Lipoxygenase [p Ser271] AntibodyBSA Free [NBP3-05439]

Western Blot: 5-Lipoxygenase [p Ser271] Antibody [NBP3-05439] - Western blot analysis of extracts of HT-29 cells, using 5-Lipoxygenase antibody (NBP3-05439) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Applications for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 5-Lipoxygenase

5 Lipoxygenase encodes a protein which, with Arachidonate 5-lipoxygenase-activating protein, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

5Lipoxygenase, 5LPG, ALOX5, LOG5, LOX5A

Gene Symbol

ALOX5

Additional 5-Lipoxygenase Products

Product Documents for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for 5-Lipoxygenase [p Ser271] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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