5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Novus Biologicals | Catalog # NBP2-37271

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4G6E3

Format

BSA Free
View all 5'-Nucleotidase/CD73 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human 5'-Nucleotidase/CD73 (AA: 30-250) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.
Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.
Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).
Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)
Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3)BSA Free [NBP2-37271]

Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free [NBP2-37271]

Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.
ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Customer Reviews for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

There are currently no reviews for this product. Be the first to review 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free and earn rewards!

Have you used 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 5'-Nucleotidase/CD73 Antibody (4G6E3) - BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: We are interested in CD73 antibodies that could react with dog, please let me know which products in your catalog you would recommend.

    A:

    We do not currently carry any CD73 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD73 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies