5-TAMRA SE
Discontinued Product
Chemical Name: 5-Carboxytetramethylrhodamine succinimidyl ester
Biological Activity
Fluorescent probe for DNA and RNA; amine-reactive. Also shown to stain telomeres. Nuclease resistant and nuclei permeable. Excitation\emission λ ~545\580 nm.Optical Data for 5-TAMRA SE
| Emission Color | Orange |
|---|---|
| λabs | 541 nm |
| λem | 567 nm |
| Extinction Coefficient (ε) | 84000 M-1cm-1 |
| Quantum Yield (φ) | 0.1 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 260 | 0.32 |
| Correction Factor 280 | 0.19 |
| Alternative For | Alexa Fluor(R) 546 |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
-
Structural evaluation of tandem hairpin pyrrole-imidazole polyamides recognizing human telomeres.
Hirata et al.
J.Am.Chem.Soc., 2014;136:11546 -
Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Kvach et al.
Bioconjug. Chem., 2009;20:1673
Product Datasheets
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