Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%), Canine (90%), Chimpanzee (96%), Feline (90%), Orangutan (96%), Porcine (90%), Primate (96%), Rabbit (90%), Sheep (90%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 1285A

Format

Azide and BSA Free
View all 53BP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant monoclonal 53BP1 Antibody (1285A) was made to amino acids 350 - 400 of human 53BP1 (NP_005648.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (96%), Gorilla (96%), Orangutan (96%), Gibbon (94%), Bovine (92%), Marmoset (92%), Canine (90%), Feline (90%), Porcine (90%), Rabbit (90%), Sheep (90%)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

>90% by SDS PAGE

Scientific Data Images for 53BP1 Antibody (1285A) - Azide and BSA Free

Western Blot: 53BP1 Antibody (1285A)Azide and BSA Free [MAB18771]

Western Blot: 53BP1 Antibody (1285A)Azide and BSA Free [MAB18771]

Western Blot: 53BP1 Antibody (1285A) [MAB18771] - Analysis of HEK293 and ZR-75 cell lysates with 1 ug/ml of h53BP1 Monoclonal Antibody followed by HRP-conjugated Anti-Rabbit IgG Antibody. The molecular weight was observed for h53BP1 at approximately 350 kDa (as indicated), and the theoretical molecular weight of the whole endogenous protein is 214 kDa. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry: 53BP1 Antibody (1285A) - Azide and BSA Free [MAB18771]

Immunocytochemistry: 53BP1 Antibody (1285A) - Azide and BSA Free [MAB18771]

Immunocytochemistry: 53BP1 Antibody (1285A) [MAB18771] - 53BP1 was detected in immersion fixed HeLa cell using 53BP1 Monoclonal Antibody at 3 ug/ml dilution. Cells were stained using the NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to nuclei.

Applications for 53BP1 Antibody (1285A) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB18771 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: 53BP1

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A.,... Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Long Name

p53 Binding Protein 1

Alternate Names

p202, TP53BP1

Gene Symbol

TP53BP1

Additional 53BP1 Products

Product Documents for 53BP1 Antibody (1285A) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 53BP1 Antibody (1285A) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

Customer Reviews for 53BP1 Antibody (1285A) - Azide and BSA Free (1)

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  • 53BP1 Antibody (1285A) - Azide and BSA Free
    Name: Maximilian Haist
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 04/03/2023
    53BP1 in Tonsil tissue section
    53BP1 Antibody (1285A) - Azide and BSA Free MAB18771
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs for 53BP1 Antibody (1285A) - Azide and BSA Free

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  • Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

    A: We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

  • Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

    A: We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

  • Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

    A: We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

  • Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

    A: We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

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