53BP1 Antibody (1285C) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80550

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bat (99%), Bovine (100%), Canine (100%), Chimpanzee (100%), Equine (100%), Feline (100%), Hamster (100%), Orangutan (100%), Porcine (100%), Primate (100%), Rabbit (100%), Rat (99%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1285C expressed in HEK293

Format

Azide and BSA Free
View all 53BP1 Primary Antibodies »

Product Specifications

Immunogen

53BP1 Antibody (1285C) was made to a synthetic peptide made to the C-terminal portion of human 53BP1 protein (between amino acids 1900-1972) [UniProt Q12888]

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gibbon (100%), Gorilla (100%), Marmoset (100%)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 53BP1 Antibody (1285C) - Azide and BSA Free

Western Blot: 53BP1 Antibody (1285C)Azide and BSA Free [NBP2-80550]

Western Blot: 53BP1 Antibody (1285C)Azide and BSA Free [NBP2-80550]

Western Blot: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - Total protein from human A431, NTERA-2, HeLa and mouse 3T3 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml 53BP1 Antibody in block solution and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight is shown on this gel at ~250 kDa and the theoretical molecular weight of the whole endogenous protein is 214 kDa. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody [1285C] NBP2-80550 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunohistochemistry-Paraffin: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunohistochemistry-Paraffin: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - 53BP1 was detected in paraffin-embedded sections of human cervical cancer tissue using Rabbit Anti-Human 53BP1 Monoclonal Antibody (clone 1285C) at 1 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Pol
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 53BP1 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) (Catalog #NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 53BP1 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) (Catalog #NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with 53BP1 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) (Catalog #NB100-690) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/ Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550]

Immunocytochemistry/Immunofluorescence: 53BP1 Antibody (1285C) - Azide and BSA Free [NBP2-80550] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-53BP1 Antibody [1285C] NBP2-80550 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for 53BP1 Antibody (1285C) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

1 ug/ml

Flow Cytometry

2.5-5 ug/ml

Immunohistochemistry

1.0 ug/ml

Immunohistochemistry-Paraffin

1.0 ug/ml

Western Blot

1.0 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 53BP1

Tumor protein p53 binding protein 1 (P53-binding protein 1 or 53BP1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase with a theoretical molecular weight of 214 kDa. Upon DNA damage, it is phosphorylated and relocalizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 plays a key role in response to DNA damage, acts as a signaling checkpoint during mitosis, and enhances TP53-mediated transcriptional activation. Originally identified as p53's transcriptional enhancing partner, 53BP1 is known as a key substrate for ataxia telangiectasia mutated (ATM) signaling, whose function to generate gamma H2AX may be partially compensated by the activity of DNA-dependent kinase (DNA-PK). 53BP1 relocalizes to discrete foci overlapping with gamma phosphorylated histone H2AX; demarcating DNA double strand breaks (DSBs) sites following exposure to radiation (1). 53BP1 functions downstream of gamma H2AX-dependent proteins that collectively establish ionizing radiation induced foci at DSBs. 53BP1 is downstream of Mre11/Rad50/NBS1 (MRN complex), MDC1, RNF8, RNF168 and HERC2 which recruit 53BP1 to the DSB site, suggesting a role in DNA repair through genomic stability maintenance (2).

References

1.Henry, E., Souissi-Sahraoui, I., Deynoux, M., Lefevre, A., Barroca, V., Campalans, A.,... Arcangeli, M. L. (2019). Human hematopoietic stem/progenitor cells display ROS-dependent long-term hematopoietic defects after exposure to low dose of ionizing radiations. Haematologica. doi:10.3324/haematol.2019.226936

2.Janoshazi, A. K., Horton, J. K., Zhao, M. L., Prasad, R., Scappini, E. L., Tucker, C. J., & Wilson, S. H. (2020). Shining light on the response to repair intermediates in DNA of living cells. DNA Repair (Amst), 85, 102749. doi:10.1016/j.dnarep.2019.102749

Long Name

p53 Binding Protein 1

Alternate Names

p202, TP53BP1

Gene Symbol

TP53BP1

Additional 53BP1 Products

Product Documents for 53BP1 Antibody (1285C) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for 53BP1 Antibody (1285C) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

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Protocols

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FAQs for 53BP1 Antibody (1285C) - Azide and BSA Free

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

    A:

    We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

  • Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

    A:

    We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

  • Q: Hello I want to ask you for an advice in case of antibodies. In our research we focus on DNA repair foci and use your Novus antibodies for phosphorylated histone H2AX (monoclonal mouse) and protein 53BP1(polyclonal rabbit). Now we want to use antobody for phosphorylated 53BP1 protein to increase specifity and I want to ask you which from your NOVUS antibodies will be the best for us.

    A:

    We currently stock just one antibody to phosphorylated 53BP1, which you can see at the following link: NB100-1803. This is guaranteed for detection of 53BP1 (pSer25) in human and mouse samples by WB, FLOW, ICC/IF,IHC-P, IP and PLA. As this antibody is a rabbit polyclonal, you would be unable to stain for both 53BP1 and its phosphorylated isoform in the same sample, unless you used an antibody from a different host for detection of total 53BP1 or used directly conjugated primaries. Our mouse monoclonal to 53BP1, with catalogue number NBP2-25028, is validated for detection of the human protein by WB, FLOW and IHC. Unfortunately we do not currently stock any other antibodies to phosphorylated 53BP1.

  • Q: We're looking for anti53BP1 antibody covalently labeled with a fluorofor preferabley Cy3. Do you make it?

    A:

    We sell 53BP1 antibodies available conjugated to DyLight 488, 550 (very similar in spectrum to Cy3) and 650. You may also purchase the unlabeled antibodies and conjugate them to the fluorophores of your choice. The catalog numbers that you may be interested in are: NB100-904R, NB100-304R, NB100-305R.

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