5T4 Antibody (6E9) - BSA Free

Novus Biologicals | Catalog # NBP3-26276

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6E9 expressed in HEK293

Format

BSA Free
View all 5T4 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human 5T4 [UniProt Q13641]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5T4 Antibody (6E9) - BSA Free

5T4 Antibody (6E9)

Immunohistochemistry: 5T4 Antibody (6E9) [NBP3-26276] -

Immunohistochemistry: 5T4 Antibody (6E9) [NBP3-26276] - Image of 5T4 Antibody (6E9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
5T4 Antibody (6E9)

Western Blot: 5T4 Antibody (6E9) [NBP3-26276] -

Western Blot: 5T4 Antibody (6E9) [NBP3-26276] - Positive Western Blot detected in: U-87 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, U-251 whole cell lysate, Mouse Brain whole cell lysate.
All lanes: 5T4 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 47 kDa
Observed band size: 50, 80 kDa

Applications for 5T4 Antibody (6E9) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: 5T4

The 5T4 gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is ass

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Gene Symbol

TPBG

Additional 5T4 Products

Product Documents for 5T4 Antibody (6E9) - BSA Free

Certificate of Analysis

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Product Specific Notices for 5T4 Antibody (6E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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