6-Hydroxydopamine hydrobromide

Tocris Bioscience | Catalog # 2547

Selective catecholaminergic neurotoxin
Tocris Bioscience

Key Product Details

Description

Selective catecholaminergic neurotoxin

Alternative Names

6-OHDA
View all Additional Dopaminergic-related Products »

Product Description

6-Hydroxydopamine hydrobromide is a selective catecholaminergic neurotoxin. Depletes brain catecholamine levels via uptake and accumulation by a transport mechanism specific to these neurons. Causes almost complete destruction of nigral dopaminergic neurons and their striatal terminals when injected into the substantia nigra of rats, producing an animal model of Parkinson's disease.

Product Specifications for 6-Hydroxydopamine hydrobromide

Molecular Weight

250.09

Formula

C8H11NO3.HBr

Storage

Store at -20°C

Chemical Name

5-(2-Aminoethyl)-1,2,4-benzenetriol hydrobromide

CAS Number

636-00-0

PubChem ID

176170

InChI Key

MLACDGUOKDOLGC-UHFFFAOYSA-N

SMILES

NCCC1=CC(O)=C(O)C=C1O.Br

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 25.01 100
DMSO 25.01 100

Preparing Stock Solutions for 6-Hydroxydopamine hydrobromide

The following data is based on the product molecular weight 250.09.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.00 mL 19.99 mL 39.99 mL
5 mM 0.80 mL 4.00 mL 8.00 mL
10 mM 0.40 mL 2.00 mL 4.00 mL
50 mM 0.08 mL 0.40 mL 0.80 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 23 publications citing the usage of this product.

Product Documents for 6-Hydroxydopamine hydrobromide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for 6-Hydroxydopamine hydrobromide

For research use only

Citations for 6-Hydroxydopamine hydrobromide

Customer Reviews for 6-Hydroxydopamine hydrobromide (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used 6-Hydroxydopamine hydrobromide?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Works well
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vivo
    Cell Line/Tissue: neuron
    Verified Customer | Posted 06/15/2020
    3-4 mg/ml
    6-Hydroxydopamine hydrobromide 2547
  • Great neurotoxic effects in dopaminergic neurons
    Name: Luiz Alexandre Magno
    Species: Mouse
    Assay Type: In Vivo
    Verified Customer | Posted 07/03/2018
    Although 6-Hydroxydopamine hydrobromide (6-OHDA.HBr #2547) dissolves very well in aqueous solutions, some tips are necessary to guarantee its stability for biological experiments. 1. When calculating the 6-OHDA concentration, take in consideration that the doses that are often reported are for the free base and not for the hydrobromide (HBr) conjugated salt. Thus, in the case of 6-OHDA.HBr (#2547), the presence of HBr has to be taken into account in making the proper solution. HBr has a molecular weight of 80.91 and represents 32.352% of the 6-OHDA.HBr (#2547) molecular mass. 2. Always dissolve the 6-OHDA salt in buffers containing antioxidant capacity. I suggest adding 1 mM ascorbic acid (#4055) into PBS. I know some colleagues that have used sodium metabisulfite as well with great success. 3. Always make fresh solutions. The 6-OHDA solution is extremely prone to oxidation, even when resuspended in antioxidant buffers. When fresh, it looks dark red. However, it turns to brown after a few hours. 4. Finally, 6-OHDA is extremely toxic. Therefore always be cautious when handling it.
    I used the 6-Hydroxydopamine (6-OHDA) to deplete dopaminergic cells from the mouse brain, which is largely used as an experimental model of Parkinson's Disease. The 6-OHDA was injected into the mouse striatum and 21 days later we observed that the dopaminergic neurons (positive for tyrosine hydroxylase immunofluorescence) were almost absent in the substantia nigra pars compacta (SNc) from the 6-OHDA injected hemisphere (on the left). As a control for analytical comparisons, we also injected PBS into the contralateral hemisphere (on the right). When this neurotoxin is injected into striatum, as in this case, there is only a slight depletion of dopaminergic neurons from the ventral tegmental area (the region located into the middle of the image). The animals injected with 6-OHDA display several motor dysfunctions and are used to study the pathology and therapies for Parkinsonian symptoms.
    6-Hydroxydopamine hydrobromide 2547

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All