Chemical Name: 5-(2-Aminoethyl)-1,2,4-benzenetriol hydrobromide
Biological ActivitySelective catecholaminergic neurotoxin. Depletes brain catecholamine levels via uptake and accumulation by a transport mechanism specific to these neurons. Causes almost complete destruction of nigral dopaminergic neurons and their striatal terminals when injected into the substantia nigra of rats, producing an animal model of Parkinson's disease.
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Effect of 6-hydroxydopamine on brain norepinephrine and dopamine: evidence for selective degeneration of catecholamine neurons.
Breese and Traylor
Autoxidation and neurotoxicity of 6-hydroxydopamine in the presence of some antioxidants: potential implication in relation to the pathogenesis of Parkinson's disease.
Soto-Otero et al.
Cell-permeable cAMP analog suppresses 6-hydroxydopamine-induced apoptosis in PC12 cells through the activation of the Akt pathway.
Fujita et al.
Brain Res., 2006;1113:10
Citations for 6-Hydroxydopamine hydrobromide
The citations listed below are publications that use Tocris products. Selected citations for 6-Hydroxydopamine hydrobromide include:
9 Citations: Showing 1 - 9
External light activates hair follicle stem cells through eyes via an ipRGC-SCN-sympathetic neural pathway.
Proc Natl Acad Sci U S A. 2018;115(29):E6880
Assessment of the Protection of Dopaminergic Neurons by an α7 Nicotinic Receptor Agonist, PHA 543613 Using [(18)F]LBT-999 in a Parkinson's Disease Rat Model.
Authors: Sérriàre Et al.
PLoS One 2015;2:61
Mitochondrial dysfunction, oxidative stress, and neurodegeneration elicited by a bacterial metabolite in a C. elegans Parkinson's model.
Authors: Ray Et al.
Cell Death Dis 2014;5:e984
Derivation and expansion using only small molecules of human neural progenitors for neurodegenerative disease modeling.
Authors: Reinhardt Et al.
Neural Plast 2013;8:e59252
ER stress inhibits neuronal death by promoting autophagy.
Authors: Fouillet Et al.
Front Med (Lausanne) 2012;8:915
Use of PC12 cells and rat superior cervical ganglion sympathetic neurons as models for neuroprotective assays relevant to Parkinson's disease.
Authors: Grau and Greene
Methods Mol Biol 2012;846:201
Suppression of bladder overactivity by adenosine A2A receptor antagonist in a rat model of Parkinson disease.
Authors: Kitta Et al.
J Urol 2012;187:1890
Rapamycin protects against neuron death in in vitro and in vivo models of Parkinson's disease.
Authors: Malagelada Et al.
J Neurosci 2010;30:1166
RTP801 is induced in Parkinson's disease and mediates neuron death by inhibiting Akt phosphorylation/activation.
Authors: Malagelada Et al.
J Neurosci 2008;28:14363
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I used the 6-Hydroxydopamine (6-OHDA) to deplete dopaminergic cells from the mouse brain, which is largely used as an experimental model of Parkinson's Disease. The 6-OHDA was injected into the mouse striatum and 21 days later we observed that the dopaminergic neurons (positive for tyrosine hydroxylase immunofluorescence) were almost absent in the substantia nigra pars compacta (SNc) from the 6-OHDA injected hemisphere (on the left). As a control for analytical comparisons, we also injected PBS into the contralateral hemisphere (on the right). When this neurotoxin is injected into striatum, as in this case, there is only a slight depletion of dopaminergic neurons from the ventral tegmental area (the region located into the middle of the image). The animals injected with 6-OHDA display several motor dysfunctions and are used to study the pathology and therapies for Parkinsonian symptoms.
Although 6-Hydroxydopamine hydrobromide (6-OHDA.HBr #2547) dissolves very well in aqueous solutions, some tips are necessary to guarantee its stability for biological experiments. 1. When calculating the 6-OHDA concentration, take in consideration that the doses that are often reported are for the free base and not for the hydrobromide (HBr) conjugated salt. Thus, in the case of 6-OHDA.HBr (#2547), the presence of HBr has to be taken into account in making the proper solution. HBr has a molecular weight of 80.91 and represents 32.352% of the 6-OHDA.HBr (#2547) molecular mass.2. Always dissolve the 6-OHDA salt in buffers containing antioxidant capacity. I suggest adding 1 mM ascorbic acid (#4055) into PBS. I know some colleagues that have used sodium metabisulfite as well with great success.3. Always make fresh solutions. The 6-OHDA solution is extremely prone to oxidation, even when resuspended in antioxidant buffers. When fresh, it looks dark red. However, it turns to brown after a few hours.4. Finally, 6-OHDA is extremely toxic. Therefore always be cautious when handling it.