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8-oxo-dG Antibody

Catalog #: 4354-MC-050
47 Citations 4 Reviews Datasheet / COA / SDS
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4354-MC-050
Product Details
Citations (47)
FAQs
Supplemental Products
Reviews (4)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

8-oxo-dG Antibody Summary

Specificity
This mouse monoclonal antibody specifically binds to 8-hydroxy-2'- deoxyguanosine within DNA in H2O2-treated cells.
Source
Monoclonal Mouse IgG2B Clone # 15A3
Purification
Protein A or G purified from ascites
Immunogen
8-oxo-dG-conjugated-KLH
Formulation
This antibody is provided as purified immunoglobulin from mouse ascites at 0.5 mg/ml in 1X PBS containing 0.1% sodium azide, 50% glycerol.
Label
Unconjugated

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Cat # D-201-C-ABS2

Applications

Recommended Concentration
Sample
ELISA
Empirical determination will be required for optimal results.
 
Immunohistochemistry
1:250 dilution
Paraffin embedded rat thymus tissue
Immunocytochemistry
1:250 dilution

H2O2 treated MCF 10A human breast epithelial cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: 8-oxo-dG

The production of 8-Hydroxyguanine (8-oxo-dG) is almost exclusively elicited by oxidative stress. Polymerases preferentially insert adenine opposite 8-oxo-dG. Therefore, oxidatively damaged adducts, without repair, are susceptible to G to T transitions.

Long Name
8-Hydroxyguanine
Alternate Names
8-OHG; 8oxodG; 8-oxo-dG

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Citations for 8-oxo-dG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

47 Citations: Showing 1 - 10
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  1. Downregulating Mitochondrial DNA Polymerase gamma in the Muscle Stimulated Autophagy, Apoptosis, and Muscle Aging-Related Phenotypes in Drosophila Adults
    Authors: M Ozaki, TD Le, YH Inoue
    Biomolecules, 2022;12(8):.
    Species: Insect - Drosophila
    Sample Types: Whole Tissue
    Applications: IHC
  2. Tumor suppressor PALB2 maintains redox and mitochondrial homeostasis in the brain and cooperates with ATG7/autophagy to suppress neurodegeneration
    Authors: Y Huo, A Sawant, Y Tan, AH Mahdi, T Li, H Ma, V Bhatt, R Yan, J Coleman, CF Dreyfus, JY Guo, MM Mouradian, E White, B Xia
    PloS Genetics, 2022;18(4):e1010138.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins
    Authors: N Kumar, AF Theil, V Roginskaya, Y Ali, M Calderon, SC Watkins, RP Barnes, PL Opresko, A Pines, H Lans, W Vermeulen, B Van Houten
    Nature Communications, 2022;13(1):974.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Oxidative DNA Damage and Cisplatin Neurotoxicity Is Exacerbated by Inhibition of OGG1 Glycosylase Activity and APE1 Endonuclease Activity in Sensory Neurons
    Authors: A Behrouzi, H Xia, EL Thompson, MR Kelley, JC Fehrenbach
    International Journal of Molecular Sciences, 2022;23(3):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  5. Bixin Attenuates High-Fat Diet-Caused Liver Steatosis and Inflammatory Injury through Nrf2/PPAR&alpha Signals
    Authors: S Tao, Y Yang, J Li, H Wang, Y Ma
    Oxidative Medicine and Cellular Longevity, 2021;2021(0):6610124.
  6. The ubiquitin E3 ligase TRIM21 promotes hepatocarcinogenesis by suppressing the p62-Keap1-Nrf2 antioxidant pathway
    Authors: F Wang, Y Zhang, J Shen, B Yang, W Dai, J Yan, S Maimouni, HQ Daguplo, S Coppola, Y Gao, Y Wang, Z Du, K Peng, H Liu, Q Zhang, F Tang, P Wang, S Gao, Y Wang, WX Ding, G Guo, F Wang, WX Zong
    Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Imbalanced autophagy causes synaptic deficits in a human model for neurodevelopmental disorders
    Authors: K Linda, EI Lewerissa, AHA Verboven, M Gabriele, M Frega, TM Klein Gunn, L Devilee, E Ulferts, M Hommersom, A Oudakker, C Schoenmake, H van Bokhov, D Schubert, G Testa, DA Koolen, BBA de Vries, N Nadif Kasr
    Autophagy, 2021;0(0):20-Jan.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. DNA damage induced during mitosis undergoes DNA repair synthesis
    Authors: V Gomez Godi, S Kabbara, A Sherman, T Wu, S Cohen, X Kong, JL Maravillas, Z Shi, D Preece, K Yokomori, MW Berns
    PLoS ONE, 2020;15(4):e0227849.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Cardiovascular Changes Related to Metabolic Syndrome: Evidence in Obese Zucker Rats
    Authors: I Martinelli, D Tomassoni, M Moruzzi, P Roy, C Cifani, F Amenta, SK Tayebati
    Int J Mol Sci, 2020;21(6):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription
    Authors: S Sengupta, H Wang, C Yang, B Szczesny, ML Hegde, S Mitra
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  11. Targeted DNA oxidation by LSD1-SMAD2/3 primes TGF-&beta1/ EMT genes for activation or repression
    Authors: A Pezone, ML Taddei, A Tramontano, J Dolcini, FL Boffo, M De Rosa, M Parri, S Stinziani, G Comito, A Porcellini, G Raugei, D Gackowski, E Zarakowska, R Olinski, A Gabrielli, P Chiarugi, EV Avvediment
    Nucleic Acids Res., 2020;0(0):.
  12. BRAF Mutant Melanoma Adjusts to BRAF/MEK Inhibitors via Dependence on Increased Antioxidant SOD2 and Increased Reactive Oxygen Species Levels
    Authors: L Yuan, R Mishra, H Patel, S Alanazi, X Wei, Z Ma, JT Garrett
    Cancers (Basel), 2020;12(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Deletion of KLF10 Leads to Stress-Induced Liver Fibrosis upon High Sucrose Feeding
    Authors: J Lee, AR Oh, HY Lee, YA Moon, HJ Lee, JY Cha
    International Journal of Molecular Sciences, 2020;22(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcription
    Authors: S Sengupta, H Wang, C Yang, B Szczesny, ML Hegde, S Mitra
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  15. Lamin A/C promotes DNA base excision repair
    Authors: S Maynard, G Keijzers, M Akbari, MB Ezra, A Hall, M Morevati, M Scheibye-K, S Gonzalo, J Bartek, VA Bohr
    Nucleic Acids Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. IL-4 and IL-17A Cooperatively Promote Hydrogen Peroxide Production, Oxidative DNA Damage, and Upregulation of Dual Oxidase 2 in Human Colon and Pancreatic Cancer Cells
    Authors: Y Wu, MM Konaté, J Lu, H Makhlouf, R Chuaqui, S Antony, JL Meitzler, MJ Difilippan, H Liu, A Juhasz, G Jiang, I Dahan, K Roy, JH Doroshow
    J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. PPAR? Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis
    Authors: CG Li, C Mahon, NM Sweeney, E Verschuere, V Kantamani, D Li, JK Hennigs, DP Marciano, I Diebold, O Abu-Halawa, M Elliott, S Sa, F Guo, L Wang, A Cao, C Guignabert, J Sollier, NP Nickel, M Kaschwich, KA Cimprich, M Rabinovitc
    Cell Rep, 2019;26(5):1333-1343.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. PRMT5 Modulates Splicing for Genome Integrity and Preserves Proteostasis of Hematopoietic Stem Cells
    Authors: DQ Tan, Y Li, C Yang, J Li, SH Tan, DWL Chin, A Nakamura-I, H Yang, T Suda
    Cell Rep, 2019;26(9):2316-2328.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Oxidative Damage Detection
  19. SIRT1 activation promotes angiogenesis in diabetic wounds by protecting endothelial cells against oxidative stress
    Authors: X Li, G Wu, F Han, K Wang, X Bai, Y Jia, Z Li, W Cai, W Zhang, L Su, D Hu
    Arch. Biochem. Biophys., 2019;661(0):117-124.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage
    Authors: WJ Yi, MY Su, Y Shi, S Jiang, SZ Xu, TC Lei
    Cell Cycle, 2018;0(0):1-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  21. Prevention of Axonal Degeneration by Perineurium Injection of Mitochondria in a Sciatic Nerve Crush Injury Model
    Authors: CC Kuo, HL Su, TL Chang, CY Chiang, ML Sheu, FC Cheng, CJ Chen, J Sheehan, HC Pan
    Neurosurgery, 2017;80(3):475-488.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. Improved neurological outcome by intramuscular injection of human amniotic fluid derived stem cells in a muscle denervation model.
    Authors: Chen C, Cheng F, Su H, Sheu M, Lu Z, Chiang C, Yang D, Sheehan J, Pan H
    PLoS ONE, 2015;10(5):e0124624.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  23. Oxidative damage increases and antioxidant gene expression decreases with aging in the mouse ovary.
    Authors: Lim J, Luderer U,
    Biol. Reprod., 2011;84(4):775-82.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Diabetes-induced oxidative DNA damage alters p53-p21CIP1/Waf1 signaling in the rat testis.
    Authors: Kilarkaje N, Al-Bader M
    Reprod Sci, 0;22(1):102-12.
  25. Mitochondrial oxidative stress-induced epigenetic modifications in pancreatic epithelial cells.
    Authors: Mishra P, Raghuram G, Jain D, Jain S, Khare N, Pathak N
    Int J Toxicol, 0;33(2):116-29.
  26. Hydroquinone increases 5-hydroxymethylcytosine formation through ten eleven translocation 1 (TET1) 5-methylcytosine dioxygenase.
    Authors: Coulter J, O'Driscoll C, Bressler J
    J Biol Chem, 0;288(40):28792-800.
  27. Sirt3 protects mitochondrial DNA damage and blocks the development of doxorubicin-induced cardiomyopathy in mice.
    Authors: Pillai V, Bindu S, Sharp W, Fang Y, Kim G, Gupta M, Samant S, Gupta M
    Am J Physiol Heart Circ Physiol, 0;310(8):H962-72.
  28. Delayed treatment with fenofibrate protects against high-fat diet-induced kidney injury in mice: the possible role of AMPK autophagy.
    Authors: Sohn M, Kim K, Uddin M, Lee G, Hwang I, Kang H, Kim H, Lee J, Ha H
    Am J Physiol Renal Physiol, 0;312(2):F323-F334.
  29. Nicotinamide enhances repair of ultraviolet radiation-induced DNA damage in human keratinocytes and ex vivo skin.
    Authors: Surjana D, Halliday G, Damian D
    Carcinogenesis, 0;34(5):1144-9.
  30. L-Lactate-Mediated Neuroprotection against Glutamate-Induced Excitotoxicity Requires ARALAR/AGC1.
    Authors: Llorente-Folch I, Rueda C, Perez-Liebana I, Satrustegui J, Pardo B
    J Neurosci, 0;36(16):4443-56.
  31. Endothelin Receptor A Antagonism Prevents Damage to Glycogen-Rich Placental Cells Following Uterine Ischemia-Reperfusion in the Rat.
    Authors: Thaete L, Khan S, Neerhof M
    Reprod Sci, 0;23(11):1518-1525.
  32. Oncogenic KRAS confers chemoresistance by upregulating NRF2.
    Authors: Tao S, Wang S, Moghaddam S, Ooi A, Chapman E, Wong P, Zhang D
    Cancer Res, 0;74(24):7430-41.
  33. Nicotinamide enhances repair of arsenic and ultraviolet radiation-induced DNA damage in HaCaT keratinocytes and ex vivo human skin.
    Authors: Thompson B, Halliday G, Damian D
    PLoS ONE, 0;10(2):e0117491.
  34. Activated protein C ameliorates diabetic nephropathy by epigenetically inhibiting the redox enzyme p66Shc.
    Authors: Bock F, Shahzad K, Wang H, Stoyanov S, Wolter J, Dong W, Pelicci P, Kashif M, Ranjan S, Schmidt S, Ritzel R, Schwenger V, Reymann K, Esmon C, Madhusudhan T, Nawroth P, Isermann B
    Proc Natl Acad Sci U S A, 0;110(2):648-53.
  35. Trajectory of Parvalbumin Cell Impairment and Loss of Cortical Inhibition in Traumatic Brain Injury.
    Authors: Hsieh T, Lee H, Hameed M, Pascual-Leone A, Hensch T, Rotenberg A
    Cereb Cortex, 0;27(12):5509-5524.
  36. Endogenous catalase delays high-fat diet-induced liver injury in mice.
    Authors: Piao L, Choi J, Kwon G, Ha H
    Korean J Physiol Pharmacol, 0;21(3):317-325.
  37. Atg7 Overcomes Senescence and Promotes Growth of BrafV600E-Driven Melanoma.
    Authors: Xie X, Koh J, Price S, White E, Mehnert J
    Cancer Discov, 0;5(4):410-23.
  38. Progression of genotype-specific oral cancer leads to senescence of cancer-associated fibroblasts and is mediated by oxidative stress and TGF-beta.
    Authors: Hassona Y, Cirillo N, Lim K, Herman A, Mellone M, Thomas G, Pitiyage G, Parkinson E, Prime S
    Carcinogenesis, 0;34(6):1286-95.
  39. Omega-3 polyunsaturated fatty acids selectively inhibit growth in neoplastic oral keratinocytes by differentially activating ERK1/2.
    Authors: Nikolakopoulou Z, Nteliopoulos G, Michael-Titus A, Parkinson E
    Carcinogenesis, 0;34(12):2716-25.
  40. DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair.
    Authors: Abdou I, Poirier G, Hendzel M, Weinfeld M
    Nucleic Acids Res, 0;43(2):875-92.
  41. Hrd1 suppresses Nrf2-mediated cellular protection during liver cirrhosis.
    Authors: Wu T, Zhao F, Gao B, Tan C, Yagishita N, Nakajima T, Wong P, Chapman E, Fang D, Zhang D
    Genes Dev, 0;28(7):708-22.
  42. Nrf2-dependent suppression of azoxymethane/dextran sulfate sodium-induced colon carcinogenesis by the cinnamon-derived dietary factor cinnamaldehyde.
    Authors: Long M, Tao S, Rojo de la Vega M, Jiang T, Wen Q, Park S, Zhang D, Wondrak G
    Cancer Prev Res (Phila), 0;8(5):444-54.
  43. Mitochondrial targeting of XJB-5-131 attenuates or improves pathophysiology in HdhQ150 animals with well-developed disease phenotypes.
    Authors: Polyzos A, Holt A, Brown C, Cosme C, Wipf P, Gomez-Marin A, Castro M, Ayala-Pena S, McMurray C
    Hum Mol Genet, 0;25(9):1792-802.
  44. Ablation of XP-V gene causes adipose tissue senescence and metabolic abnormalities.
    Authors: Chen Y, Harris R, Hatahet Z, Chou K
    Proc Natl Acad Sci U S A, 0;112(33):E4556-64.
  45. hSSB1 (NABP2/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway.
    Authors: Paquet N, Adams M, Leong V, Ashton N, Touma C, Gamsjaeger R, Cubeddu L, Beard S, Burgess J, Bolderson E, O'Byrne K, Richard D
    Nucleic Acids Res, 0;43(18):8817-29.
  46. An Essential Role of NRF2 in Diabetic Wound Healing.
    Authors: Long M, Rojo de la Vega M, Wen Q, Bharara M, Jiang T, Zhang R, Zhou S, Wong P, Wondrak G, Zheng H, Zhang D
    Diabetes, 0;65(3):780-93.
  47. Human mesenchymal stem cells with enhanced telomerase activity acquire resistance against oxidative stress-induced genomic damage.
    Authors: Trachana V, Petrakis S, Fotiadis Z, Siska E, Balis V, Gonos E, Kaloyianni M, Koliakos G
    Cytotherapy, 0;19(7):808-820.

FAQs

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Isotype Controls

Mouse IgG2B Isotype Control

MAB0041
8 Citations
1 Image
Ctrl

Mouse IgG2B Isotype Control

MAB0042
7 Citations
1 Review
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Mouse IgG2B Isotype Control

MAB004
52 Citations
6 Reviews
1 Image
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Secondary Antibodies

Donkey Anti-Mouse IgG Biotinylated Antibody

BAF018
2 Citations
WB

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

NL009
10 Citations
3 Reviews
Flow,IHC,ICC

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

NL007
19 Citations
Flow,IHC,ICC

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

NL008
10 Citations
Flow,IHC,ICC

Goat Anti-Mouse IgG Biotinylated Antibody

BAF007
4 Citations
4 Reviews
WB

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C
3 Citations
1 Review

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

F0101B
6 Citations
1 Review
1 Image
Flow

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

F0103B
5 Citations
1 Review
1 Image
Flow

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

F0102B
12 Citations
3 Reviews
1 Image
Flow

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

F0114
1 Citations
1 Image
Flow

Mouse IgG (H+L) Antibody

D-201-C-ABS2

Mouse IgG Antibody

AF007
7 Citations
WB

Mouse IgG HRP-conjugated Antibody

HAF018
13 Citations
2 Reviews
1 Image
WB

Mouse IgG HRP-conjugated Antibody

HAF007
109 Citations
11 Reviews
1 Image
WB

Mouse IgG VisUCyte HRP Polymer Antibody

VC001
2 Citations
1 Image
IHC

Mouse IgG2B Antibody

MAB0043
1 Image
Flow

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

VC002
2 Citations
2 Images
IHC

Rat Anti-Mouse IgG2B APC-conjugated Antibody

F0133
1 Citations
1 Image
Flow

Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

F0134
1 Citations
Flow

Rat Anti-Mouse IgG2B PE-conjugated Antibody

F0132
1 Citations
1 Image
Flow

Reviews for 8-oxo-dG Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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8-oxo-dG Antibody
By Anonymous on 01/03/2022
Application: IHC Sample Tested: Stomach tissue Species: Mouse
Immunohistochemistry 8-oxo-dG Antibody 4354-MC-050
8-oxo-dG Antibody
By Anonymous on 10/28/2021
Application: ICC/IF Sample Tested: Lung tissue Species: Mouse

Blue- DAPI; Green:8-oxo-dG

Immunocytochemistry/Immunofluorescence 8-oxo-dG Antibody 4354-MC-050
8-oxo-dG Antibody
By Anonymous on 08/11/2021
Application: IHC Sample Tested: prostate carcinoma Species: Human
Immunohistochemistry 8-oxo-dG Antibody 4354-MC-050
8-oxo-dG Antibody
By Anonymous on 12/28/2017
Application: IHC Sample Tested: Skin tissue Species: Human
Immunohistochemistry 8-oxo-dG Antibody 4354-MC-050