Catalog Number: 3336
Chemical Name: 6,7-Dihydro-4-hydroxy-3-(2'-hydroxy[1,1'-biphenyl]-4-yl)-6-oxo-thieno[2,3-b]pyridine-5-carbonitrile
Biological Activity
Potent, reversible AMP-activated protein kinase (AMPK) activator (EC50 = 0.8 μM) that displays selectivity towards β1 subunit-containing heterotrimers. Inhibits fatty acid synthesis (IC50 = 3.2 μM) and decreases plasma glucose and triglyceride levels in vivo. Also inhibits proliferation of mesenchymal stem cells, and impedes reprogramming of mouse embryonic fibroblasts to iPSCs.
Technical Data
  • M.Wt:
  • Formula:
  • Solubility:
    Soluble to 100 mM in DMSO and to 10 mM in ethanol
  • Purity:
  • Storage:
    Store at +4°C
  • CAS No:
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis. All Tocris products are intended for laboratory research use only.
Background References
  1. Activation of AMP-activated protein kinase (AMPK) provides a metabolic barrier to reprogramming somatic cells into stem cells.
    Vazquez-Martin et al.
    Cell Cycle, 2012;5:974
  2. Defining the mechanism of activation of AMP-activated protein kinase by the small molecule A-769662, a member of the thienopyridone family.
    Sanders et al.
    J.Biol.Chem., 2007;282:32539
  3. Thienopyridone drugs are selective activators of AMP-activated protein kinase β1-containing complexes.
    Scott et al.
    Chem.Biol., 2008;15:1220
  4. Role of AMP-activated protein kinase in regulating hypoxic survival and proliferation of mesenchymal stem cells.
    de Meester et al.
    Cardiovasc.Res., 2014;101:20
  5. Identification and characterization of a small molecule AMPK activator that treats key components of type 2 diabetes and the metabolic syndrome.
    Cool et al.
    Cell Met., 2006;3:403
  6. Metformin activates a duodenal Ampk-dependent pathway to lower hepatic glucose production in rats.
    Duca et al.
    Nat.Med., 2015;21:506

The citations listed below are publications that use Tocris products. Selected citations for A 769662 include:

Showing Results 1 - 10 of 15

  1. mTOR controls lysosome tubulation and antigen presentation in macrophages and dendritic cells.
    Authors: Saric Et al.
    Arterioscler Thromb Vasc Biol
  2. Activation of AMPK-induced autophagy ameliorates Huntington disease pathology in vitro.
    Authors: Walter Et al.
  3. Intact LKB1 activity is required for survival of dormant ovarian cancer spheroids.
    Authors: Peart Et al.
  4. Enhanced Production of Adenosine Triphosphate by Pharmacological Activation of Adenosine Monophosphate-Activated Protein Kinase Ameliorates Acetaminophen-Induced Liver Injury.
    Authors: Hwang Et al.
    J Pharmacol Exp Ther
  5. Dynamics of elongation factor 2 kinase regulation in cortical neurons in response to synaptic activity.
    Authors: Kenney Et al.
    J Neurosci
  6. Glucose and palmitate uncouple AMPK from autophagy in human aortic endothelial cells.
    Authors: Weikel Et al.
    Am J Physiol Cell Physiol
  7. A novel MEK-ERK-AMPK signaling axis controls chemokine receptor CCR7-dependent survival in human mature dendritic cells.
    Authors: López-Cotarelo Et al.
    Mol Cell
  8. Cellular energy stress induces AMPK-mediated regulation of YAP and the Hippo pathway.
    Authors: Mo Et al.
    Nat Cell Biol
  9. Bidirectional regulation of P body formation mediated by eIF4F complex formation in sensory neurons.
    Authors: Melemedjian Et al.
    Neurosci Lett
  10. AMPK-dependent degradation of TXNIP upon energy stress leads to enhanced glucose uptake via GLUT1.
    Authors: Wu Et al.
    Arthritis Rheum
  11. AMPK-dependent phosphorylation of ULK1 regulates ATG9 localization.
    Authors: Mack Et al.
  12. Lkb1 regulates organogenesis and early oncogenesis along AMPK-dependent and -independent pathways.
    Authors: Lo Et al.
    J Cell Biol
  13. Chondrocyte AMP-activated protein kinase activity suppresses matrix degradation responses to proinflammatory cytokines interleukin-1β and tumor necrosis factor α.
    Authors: Terkeltaub Et al.
    Proc Natl Acad Sci U S A
  14. 5-Aminoimidazole-4-carboxyamide-ribonucleoside (AICAR)-stimulated hepatic expression of Cyp4a10, Cyp4a14, Cyp4a31, and other peroxisome proliferator-activated receptor α-responsive mouse genes is AICAR 5'-monophosphate-dependent and AMP-activated
    Authors: Bumpus and Johnson
    J Biol Chem
  15. AMP-activated protein kinase suppresses endothelial cell inflammation through phosphorylation of transcriptional coactivator p300.
    Authors: Zhang Et al.
    Mol Cells
Expand to show all
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.

Order Details


Contact Information

Molarity Calculator
Calculate the mass, volume or concentration required for a solution

The molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) x Volume (L) x Molecular Weight (g/mol)
An example of a molarity calculation using the molarity calculator:
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?

R&D Systems Guarantee

or select another batch:


Customer Information