A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

Novus Biologicals | Catalog # NBP2-59941

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Bacteria

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SS 3A5-E2

Format

BSA Free
View all A. aeolicus BPL/BioID2 Primary Antibodies »

Product Specifications

Immunogen

GST fused to A. aeolicus BPL/BioID2

Reactivity Notes

Aquifex aeolicus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2)BSA Free [NBP2-59941]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2)BSA Free [NBP2-59941]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line stably expressing BioID2-TorsinA delta E302/3 without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS 3A5-E2 antibody. The predicted molecular weight of BioID2-Torsin A Delta E302/3 fusion protein is 65 kDa
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]

Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]

Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - A) Parental HEK293T or (B) monoclonal HEK293T cell line with BioID2 knocked-in into at least one LMNA gene alleles (resulting in BioID2 Lamin A/C fusion protein) were immunofluorescently labelled with anti-BPL R40G/BioID2 SS 3A5-E2 monoclonal antibody (green). The nuclei were visualized with Hoechst.
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]

Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free [NBP2-59941]

Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) [NBP2-59941] - Staining of HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 with SS 3A5-E2 monoclonal antibody. Cells were fixed in (A) cold methanol or (B) 4% PFA and stained with SS anti-BPL R40G/BioID2 3A5-E2 antibody (in green). The nuclei were counter-stained with Hoechst.

Applications for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1-2000
Application Notes
Positive Control: Cells overexpressing A. aeolicus BPL R40G (BioID2) construct.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for A. aeolicus BPL/BioID2 Antibody (SS 3A5-E2) - BSA Free

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Protocols

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