A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Novus Biologicals | Catalog # NBP2-59940

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 kappa Clone # SS QD1

Format

BSA Free
View all A. aeolicus BPL/BioID2 Primary Antibodies »

Product Specifications

Immunogen

GST fused to A. aeolicus BPL/BioID2

Reactivity Notes

Aquifex aeolicus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2 kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Western Blot: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Analysis in total cell lysate of parental HeLa cell line (lane 1) or HeLa polyclonal cell line expressing BioID2 tagged to TorsinA delta E302/3 (6) without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BPL R40G/BioID2 with SS QD1 antibody. The predicted molecular weight of BioID2-TorsinA delta E302/3 fusion protein is 65 kDa.
Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free [NBP2-59940]

Immunocytochemistry/ Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free [NBP2-59940]

Immunocytochemistry/Immunofluorescence: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Staining of HeLa cell line expressing doxycycline (dox) - inducible BioID2 tagged to TorsinA delta E302/3 with SS QD1 monoclonal antibody. Uninduced (-dox) or induced (+dox) cells were fixed in (A) cold methanol or (B) 4% PFA and stained with anti-BPL R40G/BioID2 SS QD1 antibody (in green). The nuclei were counter-stained with Hoechst.
Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1)BSA Free [NBP2-59940]

Simple Western: A. aeolicus BPL/BioID2 Antibody (SS QD1) [NBP2-59940] - Lane 1: lysate from untransfected HEK293 cells. Lane 2: lysate from HEK293 cells transfected with BioID2-PTEN. A specific signal for BioID2-PTEN is visible at ~94kDa.

Applications for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive Control: Cells overexpressing A. aeolicus BPL/BioID2 construct.
See Simple Western Antibody Database for Simple Western validation: separated by Size, apparent MW was 94 kDa

Reviewed Applications

Read 1 review rated 4 using NBP2-59940 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Certificate of Analysis

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Product Specific Notices for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free

Customer Reviews for A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free (1)

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  • A. aeolicus BPL/BioID2 Antibody (SS QD1)
    Name: Kurt Haas
    Application: Simple Western
    Sample Tested: HEK293 cells transfected with BioID2-PTEN
    Species: Human
    Verified Customer | Posted 11/15/2018
    1- lysate from untransfected HEK293 cells 2- lysate from HEK293 cells transfected with BioID2-PTEN A specific signal for BioID2-PTEN is visible at ~94kDa.
    For Simple Western (see review image): Lysates were diluted to 0.3125mg/mL before combining with 5x Fluorescent Master Mix and subjecting samples to Simple Western using default assay parameters (Protein Simple). Primary antibody to BioID2 (NBP2-59940) that was used in assay was diluted to 5ug/mL (1:200) in Antibody Diluent 2 (Protein Simple). Product review code: SW-1WB
    A. aeolicus BPL/BioID2 Antibody (SS QD1) - BSA Free NBP2-59940

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