A2BP1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92816

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RBFOX1 (NP_665900.1). MLASQGVLLHPYGVPMIVPAAPYLPGLIQGNQEAAAAPDTMAQPYASAQFAPPQNGIPAEYTAPHPHPAPEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQTDDAAPTDGQPQTQPSENT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit A2BP1 Antibody - Azide and BSA Free (NBP2-92816) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for A2BP1 Antibody - Azide and BSA Free

A2BP1 Antibody - Azide and BSA Free

Western Blot: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] -

Western Blot: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] - Western blot analysis of various lysates, using A2BP1 antibody at 1:800 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
A2BP1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] -

Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] - Immunofluorescence analysis of mouse brain cells using A2BP1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
A2BP1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] -

Immunocytochemistry/ Immunofluorescence: A2BP1 Antibody - Azide and BSA Free [NBP2-92816] - Immunofluorescence analysis of rat brain cells using A2BP1 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Applications for A2BP1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: A2BP1

Ataxin-2 binding protein 1 has an RNP motif that is highly conserved among RNA-binding proteins. This protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the gene product of the SCA2 gene which causes familial neurodegenerative diseases. Ataxin-2 binding protein 1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been found but their full length nature has not been determined. (provided by RefSeq)

Alternate Names

2BP1, A2BP, A2BP1 Hexaribonucleotide-binding protein 1, Ataxin-2-binding protein 1, FOX-1, FOX1 hexaribonucleotide binding protein 1, Fox-1 homolog A, HRNBP1 ataxin 2-binding protein 1, RNA binding protein fox-1 homolog 1, RNA binding protein, fox-1 homolog (C. elegans) 1

Gene Symbol

RBFOX1

Additional A2BP1 Products

Product Documents for A2BP1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for A2BP1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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