A33/GPA33 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89332

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all A33 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQFCNITVA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for A33/GPA33 Antibody - BSA Free

Western Blot: A33/GPA33 Antibody [NBP1-89332]

Western Blot: A33/GPA33 Antibody [NBP1-89332]

Western Blot: A33/GPA33 Antibody [NBP1-89332] - Analysis in human colon tissue.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human colon shows high expression.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Analysis in human colon and liver tissues using NBP1-89332 antibody. Corresponding GPA33 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332]

Immunohistochemistry-Paraffin: A33/GPA33 Antibody [NBP1-89332] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for A33/GPA33 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: A33

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Gene Symbol

GPA33

Additional A33 Products

Product Documents for A33/GPA33 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for A33/GPA33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for A33/GPA33 Antibody - BSA Free

Customer Reviews for A33/GPA33 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review A33/GPA33 Antibody - BSA Free and earn rewards!

Have you used A33/GPA33 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies