AAAS Antibody (5A1) - Azide and BSA Free

Novus Biologicals | Catalog # H00008086-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5A1

Format

Azide and BSA Free
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Product Specifications

Immunogen

AAAS (NP_056480, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCSLGLFPPPPPRGQVTLYEHNNELVTGSSYESPPPDFRGQWINLPVLQLTKDPLKTPGRLDHGTRTAFIHHREQVWKRCINIWRDVGLFGVLNEIANSE

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

AAAS - achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for AAAS Antibody (5A1) - Azide and BSA Free

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02] - AAAS monoclonal antibody (M02), clone 5A1. Analysis of AAAS expression in NIH/3T3.
Immunohistochemistry-Paraffin: AAAS Antibody (5A1) [H00008086-M02]

Immunohistochemistry-Paraffin: AAAS Antibody (5A1) [H00008086-M02]

Immunohistochemistry-Paraffin: AAAS Antibody (5A1) [H00008086-M02] - Analysis of monoclonal antibody to AAAS on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02] - Western Blot analysis of AAAS expression in HeLa ( Cat # L013V1 ).
Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02] - AAAS monoclonal antibody (M02), clone 5A1. Analysis of AAAS expression in PC-12.
Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02] - AAAS monoclonal antibody (M02), clone 5A1. Analysis of AAAS expression in Jurkat.
Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02]

Western Blot: AAAS Antibody (5A1) [H00008086-M02] - Analysis of AAAS expression in transfected 293T cell line by AAAS monoclonal antibody (M02), clone 5A1.Lane 1: AAAS transfected lysate (Predicted MW: 59.6 KDa).Lane 2: Non-transfected lysate.
ELISA: AAAS Antibody (5A1) [H00008086-M02] - Detection limit for recombinant GST tagged AAAS is approximately 1ng/ml as a capture antibody.

Applications for AAAS Antibody (5A1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AAAS

AAAS plays a role in the normal development of the peripheral and central nervous system

Alternate Names

AAA, AAASb, achalasia, adrenocortical insufficiency, alacrimia, achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A), ADRACALA, ADRACALIN, aladin, Allgrove, triple-A, DKFZp586G1624

Entrez Gene IDs

8086 (Human)

Gene Symbol

AAAS

OMIM

231550 (Human)

Additional AAAS Products

Product Documents for AAAS Antibody (5A1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for AAAS Antibody (5A1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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