Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012 expressed in HEK293

Format

Azide and BSA Free
View all AARSD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human AARSD1 (Uniprot#: Q9BTE6-2; Thr176-Glu525).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for AARSD1 Antibody (012) - Azide and BSA Free

Western Blot: AARSD1 Antibody (012) [NBP2-90276]

Western Blot: AARSD1 Antibody (012) [NBP2-90276]

Western Blot: AARSD1 Antibody (012) [NBP2-90276] - Anti-AARSD1 rabbit monoclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 50 kDa.
AARSD1 Antibody (012) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: AARSD1 Antibody (012) [NBP2-90276] -

Immunocytochemistry/ Immunofluorescence: AARSD1 Antibody (012) [NBP2-90276] - Immunofluorescence staining of Human AARSD1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90276 (1:60) at 4 overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Applications for AARSD1 Antibody (012) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AARSD1

Alternate Names

alanyl-tRNA editing protein Aarsd1, alanyl-tRNA synthetase domain containing 1, Alanyl-tRNA synthetase domain-containing protein 1, MGC2744

Gene Symbol

AARSD1

Additional AARSD1 Products

Product Documents for AARSD1 Antibody (012) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for AARSD1 Antibody (012) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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