ABAT Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-21598

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABAT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ABAT. The exact sequence is proprietary.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ABAT Antibody - BSA Free

Western Blot: ABAT Antibody [NBP2-21598]

Western Blot: ABAT Antibody [NBP2-21598]

Western Blot: ABAT Antibody [NBP2-21598] - 30 ug SK-N-SH whole cell lysate/extract 7. 5 % SDS-PAGE ABAT antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598]

Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598]

Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - Mouse kidney. ABAT antibody dilution: 1:500.
ABAT Antibody

Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] -

Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] - ABAT antibody detects ABAT protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ABAT stained by ABAT antibody (NBP2-21598) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ABAT Antibody - BSA Free

Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] -

ABAT antibody detects ABAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: ABAT protein stained by ABAT antibody (NBP2-21598) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
ABAT Antibody - BSA Free

Immunohistochemistry-Frozen: ABAT Antibody [NBP2-21598] -

ABAT antibody detects ABAT protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: ABAT protein stained by ABAT antibody (NBP2-21598) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.
Blue: Fluoroshield with DAPI.
ABAT Antibody - BSA Free

Western Blot: ABAT Antibody [NBP2-21598] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ABAT antibody (NBP2-21598) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ABAT Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ABAT Antibody [NBP2-21598] -

ABAT antibody detects ABAT protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: ABAT protein stained by ABAT antibody (NBP2-21598) diluted at 1:500.
Blue: Hoechst 33342 staining.
ABAT Antibody - BSA Free

Immunohistochemistry-Paraffin: ABAT Antibody [NBP2-21598] -

ABAT antibody detects ABAT protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ABAT antibody (NBP2-21598) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for ABAT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ABAT

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq]

Long Name

4-aminobutyrate aminotransferase, mitochondrial

Alternate Names

EC 2.6.1.19, EC 2.6.1.22, GABA transaminase, GABA-AT, GABA-T, GABAT, L-AIBAT

Gene Symbol

ABAT

UniProt

P80404 (Human)

Additional ABAT Products

Product Documents for ABAT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ABAT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for ABAT Antibody - BSA Free

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Protocols

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FAQs

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