ABCB11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92689

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABCB11 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human ABCB11 (NP_003733.2). RFFQLLDRQPPISVYNTAGEKWDNFQGKIDFVDCKFTYPSRPDSQVLNGLSVSISPGQTLAFVGSSGCGKSTSIQLLERFYDPDQGKVMIDGHDSKKVNVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ABCB11 Antibody - BSA Free (NBP2-92689) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ABCB11 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ABCB11 Antibody - Azide and BSA Free [NBP2-92689]

Immunocytochemistry/ Immunofluorescence: ABCB11 Antibody - Azide and BSA Free [NBP2-92689]

Immunocytochemistry/Immunofluorescence: ABCB11 Antibody [NBP2-92689] - Immunofluorescence analysis of HeLa cells using ABCB11 antibody (NBP2-92689) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ABCB11 Antibody - Azide and BSA Free [NBP2-92689]

Immunocytochemistry/ Immunofluorescence: ABCB11 Antibody - Azide and BSA Free [NBP2-92689]

Immunocytochemistry/Immunofluorescence: ABCB11 Antibody [NBP2-92689] - Analysis of NIH-3T3 cells using ABCB11. Blue: DAPI for nuclear staining.
ABCB11 Antibody - Azide and BSA Free

Western Blot: ABCB11 Antibody - Azide and BSA Free [NBP2-92689] -

Western Blot: ABCB11 Antibody - Azide and BSA Free [NBP2-92689] - Western blot analysis of lysates from Mouse liver, using ABCB11 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for ABCB11 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ABCB11

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. (provided by RefSeq)

Alternate Names

ABC member 16, MDR/TAP subfamily, ABC16, ATP-binding cassette sub-family B member 11, ATP-binding cassette, sub-family B (MDR/TAP), member 11, bile salt export pump, BSEP, BSEPPFIC2, EC 3.6.3, EC 3.6.3.44, PFIC-2, PGY4, progressive familial intrahepatic cholestasis 2, sister p-glycoprotein, SPGPBRIC2

Gene Symbol

ABCB11

Additional ABCB11 Products

Product Documents for ABCB11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ABCB11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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