ABCB5 Antibody (CL13618) - BSA Free

Novus Biologicals | Catalog # NBP3-43642

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13618

Format

BSA Free
View all ABCB5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q2M3G0, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (81%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ABCB5 Antibody (CL13618) - BSA Free

ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Analysis in human epididymis and tonsil tissues using NBP3-43642 antibody. Corresponding ABCB5 RNA-seq data are presented for the same tissues.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]-Staining of human breast shows strong membranous positivity in glandular cells.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Staining of human epididymis shows strong membranous positivity in glandular cells.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Staining of human eye retina shows strong positivity in the nerve fiber layer.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Staining of human prostate shows moderate membranous positivity in the basal layer of glandular cells.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Staining of human tonsil shows no positivity in lymphoid cells as expected.
ABCB5 Antibody (CL13618) [Unconjugated] Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Immunohistochemistry-Paraffin: ABCB5 Antibody (CL13618) [NBP3-43642]

Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.

Applications for ABCB5 Antibody (CL13618) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABCB5

ATP-Binding Cassette proteins comprise a superfamily of transporters with a wide variety of substrates. ABCB5 is a novel member of the human P-glycoprotein family. It functions as a determinant of membrane potential and regulator of cell fusion in physiologic skin progenitor cells. Cell fusion is thought to contribute to tissue differentiation. ABCB5 is predominantly expressed in melanoma cells and is a novel molecular marker for a distinct subset of chemoresistant stem cell phenotype-expressing tumor cells among human epidermal melanocytes. It has been identified as a novel drug transporter in human malignant melanoma. ABCB5 is a rhodamine efflux transporter. This antibody product is intended to be used to confirm cellular localization and expression level of ABCB5.

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 5

Alternate Names

ABCB5 P-gp, ABCB5alpha, ABCB5beta, ATP-binding cassette protein, ATP-binding cassette sub-family B member 5, ATP-binding cassette, sub-family B (MDR/TAP), member 5, EC 3.6.3, EC 3.6.3.44, EST422562, P-glycoprotein ABCB5

Gene Symbol

ABCB5

Additional ABCB5 Products

Product Documents for ABCB5 Antibody (CL13618) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ABCB5 Antibody (CL13618) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ABCB5 Antibody (CL13618) - BSA Free

There are currently no reviews for this product. Be the first to review ABCB5 Antibody (CL13618) - BSA Free and earn rewards!

Have you used ABCB5 Antibody (CL13618) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies