ABHD4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83711

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ABHD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ABHD4 Antibody - BSA Free

Western Blot: ABHD4 Antibody [NBP1-83711]

Western Blot: ABHD4 Antibody [NBP1-83711]

Western Blot: ABHD4 Antibody [NBP1-83711] - Analysis in control (vector only transfected HEK293T lysate) and ABHD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ABHD4 Antibody [NBP1-83711]

Immunocytochemistry/ Immunofluorescence: ABHD4 Antibody [NBP1-83711]

Immunocytochemistry/Immunofluorescence: ABHD4 Antibody [NBP1-83711] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-83711]

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-83711]

Immunohistochemistry-Paraffin: ABHD4 Antibody [NBP1-83711] - Staining of human cerebellum shows moderate cytoplasmic positivity in purkinje cells.

Applications for ABHD4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ABHD4

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis ofN-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains fromN-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate forN-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acylchains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine,lysophosphatidylethanolamine and lysophosphatidylserine

Alternate Names

ABH4, abhydrolase domain containing 4, abhydrolase domain-containing protein 4, Alpha/beta-hydrolase 4, EC 3.1.1.-, FLJ12816, Lyso-N-acylphosphatidylethanolamine lipase

Gene Symbol

ABHD4

Additional ABHD4 Products

Product Documents for ABHD4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ABHD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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