ACAA1 Antibody (CL2660) - BSA Free
Novus Biologicals | Catalog # NBP2-42383
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL2660
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P09110, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected to cross react based on sequence identity: Mouse (88%), Rat (87%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for ACAA1 Antibody (CL2660) - BSA Free
![Western Blot: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Western-Blot-NBP2-42383-img0001.jpg "Western Blot: ACAA1 Antibody (CL2660) [NBP2-42383]")
Western Blot: ACAA1 Antibody (CL2660) [NBP2-42383]
Western Blot: ACAA1 Antibody (CL2660) [NBP2-42383] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line U-251![Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunocytochemistry-Immunofluorescence-NBP2-42383-img0007.jpg "Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunocytochemistry/ Immunofluorescence: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunocytochemistry/Immunofluorescence: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of CACO-2 cells using the Anti-ACAA1 monoclonal antibody, showing specific staining in peroxisomes in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunohistochemistry-NBP2-42383-img0006.jpg "Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human skeletal muscle shows granular cytoplasmic immunoreactivity.![Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunohistochemistry-NBP2-42383-img0002.jpg "Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.![Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunohistochemistry-NBP2-42383-img0003.jpg "Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.![Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunohistochemistry-NBP2-42383-img0004.jpg "Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human prostate shows granular cytoplasmic immunoreactivity in glandular cells.![Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]](https://resources.rndsystems.com/images/products/ACAA1-Antibody-CL2660-Immunohistochemistry-NBP2-42383-img0005.jpg "Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]")
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383]
Immunohistochemistry: ACAA1 Antibody (CL2660) [NBP2-42383] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.Applications for ACAA1 Antibody (CL2660) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ACAA1
Alternate Names
Acetyl-CoA acyltransferase, acetyl-CoA acyltransferase 1, EC 2.3.1, peroxisomal
Gene Symbol
ACAA1
UniProt
Additional ACAA1 Products
Product Documents for ACAA1 Antibody (CL2660) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ACAA1 Antibody (CL2660) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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