ACAD8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47559

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACAD8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LSFGKKEKKVGWNSQPTRAVIFEDCAVPVANRIGSEGQGFLIAVRGLNGGRINIASCSLGAAHASVILTRDHLNVRKQFGEPLASNQYLQFTLADMA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACAD8 Antibody - BSA Free

Western Blot: ACAD8 Antibody [NBP2-47559]

Western Blot: ACAD8 Antibody [NBP2-47559]

Western Blot: ACAD8 Antibody [NBP2-47559] - Analysis in control (vector only transfected HEK293T lysate) and ACAD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining in human kidney and lymph node tissues using NBP2-47559 antibody. Corresponding ACAD8 RNA-seq data are presented for the same tissues.
Western Blot: ACAD8 Antibody [NBP2-47559]

Western Blot: ACAD8 Antibody [NBP2-47559]

Western Blot: ACAD8 Antibody [NBP2-47559] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HEL
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human lymph node shows weak granular cytoplasmic positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559]

Immunohistochemistry-Paraffin: ACAD8 Antibody [NBP2-47559] - Staining of human prostate shows moderate granular cytoplasmic positivity in glandular cells.

Applications for ACAD8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAD8

Isobutyryl-CoA dehydrogenase (EC 1.3.99), encoded by the ACAD8 gene, catalyzes the third step of the degradation of the branched chain amino acid valine (Nguyen et al., 2002 [PubMed 12359132]).[supplied by OMIM]

Alternate Names

ACAD-8, Activator-recruited cofactor 42 kDa component, Acyl-CoA dehydrogenase family member 8, acyl-CoA dehydrogenase family, member 8, acyl-Coenzyme A dehydrogenase family, member 8, ARC42, EC 1.3.99, EC 1.3.99.-, FLJ22590, IBD, isobutyryl-CoA dehydrogenase, mitochondrial

Gene Symbol

ACAD8

Additional ACAD8 Products

Product Documents for ACAD8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACAD8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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