ACADM Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89292

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACADM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACADM Antibody - BSA Free

Western Blot: ACADM Antibody [NBP1-89292]

Western Blot: ACADM Antibody [NBP1-89292]

Western Blot: ACADM Antibody [NBP1-89292] - Staining of human kidney shows high expression.
Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP1-89292]

Immunocytochemistry/ Immunofluorescence: ACADM Antibody [NBP1-89292]

Immunocytochemistry/Immunofluorescence: ACADM Antibody [NBP1-89292] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human kidney, liver, lymph node and skin using Anti-ACADM antibody NBP1-89292 (A) shows similar protein distribution across tissues to independent antibody NBP1-89293 (B).
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human skin shows low expression as expected.
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining in human kidney and skin tissues using anti-ACADM antibody. Corresponding ACADM RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292]

Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Applications for ACADM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACADM

ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.

Alternate Names

ACAD1, acyl-CoA dehydrogenase, C-4 to C-12 straight chain, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, EC 1.3.99, EC 1.3.99.3, FLJ18227, FLJ99884, MCADFLJ93013, MCADH, medium-chain specific acyl-CoA dehydrogenase, mitochondrial

Gene Symbol

ACADM

Additional ACADM Products

Product Documents for ACADM Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACADM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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