ACADSB Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15237

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACADSB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACADSB. The exact sequence is proprietary.

Reactivity Notes

Rat (81%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACADSB Antibody - BSA Free

Western Blot: ACADSB Antibody [NBP2-15237]

Western Blot: ACADSB Antibody [NBP2-15237]

Western Blot: ACADSB Antibody [NBP2-15237] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ACADSB antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-15237]

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-15237]

Immunohistochemistry-Paraffin: ACADSB Antibody [NBP2-15237] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: ACADSB Antibody [NBP2-15237]

Western Blot: ACADSB Antibody [NBP2-15237]

Western Blot: ACADSB Antibody [NBP2-15237] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE; antibody diluted at 1:10000.
ACADSB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ACADSB Antibody [NBP2-15237] -

SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: SBCAD protein stained by SBCAD antibody (NBP2-15237) diluted at 1:5000.
Blue: Hoechst 33342 staining.
ACADSB Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ACADSB Antibody [NBP2-15237] -

SBCAD antibody detects SBCAD protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: SBCAD protein stained by SBCAD antibody (NBP2-15237) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for ACADSB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:5000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACADSB

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq]

Long Name

Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial

Alternate Names

2-MEBCAD, EC 1.3.8.5, SBCAD

Gene Symbol

ACADSB

UniProt

P45954 (Human)

Additional ACADSB Products

Product Documents for ACADSB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ACADSB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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