ACAT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89525

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACAT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLS

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24506864). Rat (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACAT2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining in human liver and skeletal muscle tissues using NBP1-89525 antibody. Corresponding ACAT2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human gastrointestinal, liver, skeletal muscle and testis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar protein distribution across tissues to independent antibody NBP1-89526 (B).
Western Blot: ACAT2 Antibody [NBP1-89525]

Western Blot: ACAT2 Antibody [NBP1-89525]

Western Blot: ACAT2 Antibody [NBP1-89525] - Analysis using Anti-ACAT2 antibody NBP1-89525 (A) shows similar pattern to independent antibody NBP1-85967 (B).
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP1-89525] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Applications for ACAT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACAT2

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Entrez Gene IDs

39 (Human)

Gene Symbol

ACAT2

UniProt

Q9BWD1 (Human)

Additional ACAT2 Products

Product Documents for ACAT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ACAT2 Antibody - BSA Free

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Protocols

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FAQs

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