Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACAT2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 150 to 200 of human Acetyl-CoA Acetyltransferase 2 using the numbering given in entry NP_005882.2 (GeneID 39).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ACAT2 Antibody - BSA Free

Western Blot: ACAT2 Antibody [NBP2-32198]

Western Blot: ACAT2 Antibody [NBP2-32198]

Western Blot: ACAT2 Antibody [NBP2-32198] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-ACAT2 antibody NBP2-32198 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198]

Immunohistochemistry-Paraffin: ACAT2 Antibody [NBP2-32198] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ACAT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Applications for ACAT2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:2000 - 1:10000
Application Notes
Immunoprecipitation Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ACAT2

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

Long Name

Acetyl-CoA acetyltransferase, cytosolic

Alternate Names

ACTL, EC 2.3.1.9

Gene Symbol

ACAT2

UniProt

NP_005882.2 (Human)

Additional ACAT2 Products

Product Documents for ACAT2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACAT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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