ACBD3 Antibody (OTI3A1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72149

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A1

Format

Azide and BSA Free
View all ACBD3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human ACBD3(NP_073572) produced in HKE293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACBD3 Antibody (OTI3A1) - Azide and BSA Free

Western Blot: ACBD3 Antibody (OTI3A1)Azide and BSA Free [NBP2-72149]

Western Blot: ACBD3 Antibody (OTI3A1)Azide and BSA Free [NBP2-72149]

Western Blot: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Human pancreas tissue using anti-ACBD3 mouse monoclonal antibody.
Flow Cytometry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Flow Cytometry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Flow Cytometry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-ACBD3 antibody, and then analyzed by flow cytometry.
Western Blot: ACBD3 Antibody (OTI3A1)Azide and BSA Free [NBP2-72149]

Western Blot: ACBD3 Antibody (OTI3A1)Azide and BSA Free [NBP2-72149]

Western Blot: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Human breast tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Human colon tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Human liver tissue using anti-ACBD3 mouse monoclonal antibody.
Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149]

Immunohistochemistry: ACBD3 Antibody (OTI3A1) - Azide and BSA Free [NBP2-72149] - Staining of paraffin-embedded Human prostate tissue using anti-ACBD3 mouse monoclonal antibody.

Applications for ACBD3 Antibody (OTI3A1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ACBD3

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation.

Alternate Names

acyl-CoA binding domain containing 3, Acyl-CoA-binding domain-containing protein 3, acyl-Coenzyme A binding domain containing 3, GCP60Golgi phosphoprotein 1, GOCAP1Peripheral benzodiazepine receptor-associated protein PAP7, golgi complex associated protein 1, 60kDa, Golgi complex-associated protein 1, Golgi resident protein GCP60, GOLPH1PBR- and PKA-associated protein 7, PAP7, PBR associated protein, peripherial benzodiazepine receptor associated protein, PKA (RIalpha)-associated protein

Gene Symbol

ACBD3

Additional ACBD3 Products

Product Documents for ACBD3 Antibody (OTI3A1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACBD3 Antibody (OTI3A1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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