ACE/CD143 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-91666

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACE/CD143 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFVEEYDRTSQVVWNEYAEANWNYNTNITTETSKILLQKNMQIANHTLKYGTQARKFDVNQLQNTTIKRIIKKVQDLERAALPAQELEEYNKILLDMETTYSVATVCHPNGSCLQLEPDLTNVMATSRKYEDLLWAWE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ACE/CD143 Antibody - BSA Free (NBP1-91666) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ACE/CD143 Antibody - BSA Free

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666] - Staining in human small intestine and liver tissues using NBP1-91666 antibody. Corresponding ACE RNA-seq data are presented for the same tissues.
Western Blot: ACE/CD143 Antibody [NBP1-91666]

Western Blot: ACE/CD143 Antibody [NBP1-91666]

Western Blot: ACE/CD143 Antibody [NBP1-91666] - Analysis in control (vector only transfected HEK293T lysate) and ACE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666] - Staining of human lung shows moderate membranous positivity in pneumocytes.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666]

Immunohistochemistry-Paraffin: ACE/CD143 Antibody [NBP1-91666] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.
ACE/CD143 Antibody - BSA Free Western Blot: ACE/CD143 Antibody - BSA Free [NBP1-91666]

Western Blot: ACE/CD143 Antibody - BSA Free [NBP1-91666]

Analysis in control (vector only transfected HEK293T lysate) and ACE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for ACE/CD143 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ACE/CD143

ACE converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilatator. ACE exists in two forms, a 170KD somatic form and a 90KD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

Long Name

Angiotensin I Converting Enzyme

Alternate Names

CD143, DCP1, Kininase II

Gene Symbol

ACE

Additional ACE/CD143 Products

Product Documents for ACE/CD143 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ACE/CD143 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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