Acetyl-Lysine Antibody (1C6)

Novus Biologicals | Catalog # NB100-74339

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Dot Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1C6
View all Acetyl-Lysine Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Acetyl-Lysine Antibody (1C6)

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of Acetyl-Lysine in HeLA cells.
Immunocytochemistry/ Immunofluorescence: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunocytochemistry/ Immunofluorescence: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunocytochemistry/Immunofluorescence: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of lysine acetylated proteins (green) in HeLa cells left untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels).
Chromatin Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of chromatin proteins with Acetyl-Lysine was performed using cross-linked chromatin from 4-10^6 LNCaP cells. Immunoprecipitation was performed using the Magnetic ChIP kit with 10ug of an Acetyl-Lysine monoclonal antibody. Quantitation of immunoprecipitated GAPDH promoter sequence is presented as fold enrichment of the acetyl lysine monoclonal antibody versus non-specific IgG.
Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of Acetyl-Lysine was performed by loading various amounts of acetylated BSA or non-acetylated BSA per well and 10ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl-Lysine monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Western Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of lysine acetylated proteins from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours was performed by loading 50 ug of the indicated whole cell lysates per well and 10ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl-Lysine monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
Dot Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Specific detection of lysine acetylated histones using an Acetyl-Lysine monoclonal antibody. Histone peptides containing 384 different modification combinations spotted in duplicate on a peptide array were used according to the manufacturer instructions. A schematic and table with representative results (positive detection in red, negative in blue) are shown. Result interpretation and quantification were performed using the manufacturer software. Chemiluminescent detection was performed.
Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Immunoprecipitation of acetylated alpha-Tubulin was performed.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of an Acetyl-Lysine monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2000 for at least 1 hour.
Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339]

Immunoprecipitation: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Analysis of acetylated Histone H3 (Lys9) was performed using whole cell lysates from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500ug of the indicated lysate with 3ug of an Acetyl-Lysine mAb overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hr. The membrane was probed with an Acetyl-Histone H3 (Lys9) mAb at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
Dot Blot: Acetyl-Lysine Antibody (1C6) [NB100-74339] - Specific detection of lysine acetylated histones using an Acetyl-Lysine monoclonal antibody. Histone peptides containing 384 different modification combinations spotted in duplicate on a peptide array were used according to the manufacturer's instructions (Active Motif). Representative results showing specific detection of acetylated Histone H4 K20 (blue circles, panel A) and H3 K9 (blue circles, panel B). Note that Histone H4 K20me2/K20me3 (red and yellow circles, panel A) and H3 K9me2/K9m3 (red and yellow circles, panel B) are not detected by the Acetyl-Lysine monoclonal antibody.

Applications for Acetyl-Lysine Antibody (1C6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10 ug

Dot Blot

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunoprecipitation

3 ug

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 2 reviews rated 5 using NB100-74339 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/mL BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Acetyl-Lysine

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

Alternate Names

acetyl Lysine, AC-LYS-OH, Lysine, N-acetyl lysine

Additional Acetyl-Lysine Products

Product Documents for Acetyl-Lysine Antibody (1C6)

Certificate of Analysis

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Product Specific Notices for Acetyl-Lysine Antibody (1C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Acetyl-Lysine Antibody (1C6)

Customer Reviews for Acetyl-Lysine Antibody (1C6) (2)

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  • Acetylated Lysine Antibody (1C6)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Hepatic stellate cells
    Species: Mouse
    Verified Customer | Posted 11/05/2021
    Hepatic stellate cells
    Primary antibody dilution 1:100
    Acetyl-Lysine Antibody (1C6) NB100-74339
  • Name: Michaela Rekow
    Application: Western Blot
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 01/14/2016

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