ACF1 Antibody

Novus Biologicals | Catalog # NB100-61041

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all ACF1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 725 and 775 of human ATP-dependent chromatin remodeling protein 1 (Bromodomain adjacent to zinc finger domain protein 1A ) using the numbering given in entry NP_038476.2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

179 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACF1 Antibody

Western Blot: ACF1 Antibody [NB100-61041]

Western Blot: ACF1 Antibody [NB100-61041]

Western Blot: ACF1 Antibody [NB100-61041] - Detection of Human ACF1/BAZ1A on HeLa whole cell lysates (5, 15, and 50 microgram for WB; 1 mg for IP, 20% of IP loaded) using NB100-6104. ACF1/BAZ1A was also immunoprecipitated by rabbit anti-ACF1/BAZ1A antibody NB100-61042.

Applications for ACF1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ACF1

ACF1/BAZ1A is a subunit of the ACF chromatin-remodeling complex and the ISWI CHRAC remodeling complex. In the ACF complex, ACF1/BAZ1A functions to influence the nucleosome remodeling activity of the SNF2h ATPase. As part of the CHRAC complex, ACF1/BAZ1A may function to target CHRAC to heterochromatin. ACF1 has also been shown to interact with the nuclear receptor corepressor protein (N-CoR) to facilitate the repression of transcription by unliganded nuclear receptors. Alternate names for ACF1/BAZ1A include ATP-utilizing chromatin assembly and remodeling factor1, hACF1, bromodomain adjacent to zinc finger domain protein 1A, Williams syndrome transcription factor-related chromatin-remodeling factor 180, WCRF180, hWALp1, and CHRAC subunit ACF1.

Alternate Names

ACF1hWALp1, ATP-dependent chromatin remodeling protein, ATP-dependent chromatin-remodeling protein, ATP-utilizing chromatin assembly and remodeling factor 1, bromodomain adjacent to zinc finger domain protein 1A, bromodomain adjacent to zinc finger domain, 1A, CHRAC subunit ACF1, hACF1FLJ14383, WALp1, WCRF180DKFZp586E0518, Williams syndrome transcription factor-related chromatin-remodeling factor 180

Entrez Gene IDs

11177 (Human)

Gene Symbol

BAZ1A

UniProt

Q9NRL2 (Human)

Additional ACF1 Products

Product Documents for ACF1 Antibody

Certificate of Analysis

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Product Specific Notices for ACF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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