Aconitase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32781

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Predicted:

Bovine (96%), Chicken (92%), Porcine (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (86%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aconitase 2 Antibody - BSA Free

Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [C1C3] diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody [NBP1-32781]

Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody [NBP1-32781]

Immunocytochemistry/Immunofluorescence: Aconitase 2 Antibody [NBP1-32781] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [C1C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP1-32781] - Paraffin-embedded mouse duodenum. Aconitase 2 antibody [C1C3] dilution: 1:500.
Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (20 ug of whole cell lysate) A: mouse brain 7. 5% SDS PAGE, antibody diluted at 1:20000.
Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781]

Western Blot: Aconitase 2 Antibody [NBP1-32781] - Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE, antibody diluted at 1:10000.
Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781]

Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781]

Immunoprecipitation: Aconitase 2 Antibody [NBP1-32781] - HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [C1C3]. Western blot analysis was performed using Aconitase 2 antibody [C1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Aconitase 2 Antibody - BSA Free

Western Blot: Aconitase 2 Antibody - BSA Free [NBP1-32781] -

SOD2 protein level evaluated after H2O2 treatment. (A) Western blot of SOD2 and Aconitase 2 (ACO2, used as a control) in HFFF2 and HF-TERT cells at 1 or 3 h of recovery after H2O2 treatment. (B) Quantification of SOD2 protein level in both cell lines after H2O2 treatment. Data represent mean +/- SEM. Statistical analysis is performed between HF-TERT and HFFF2 untreated cells, or between treated and untreated HF-TERT. * p < 0.05; *** p < 0.001 by t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36901881), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Aconitase 2 Antibody - BSA Free

Western Blot: Aconitase 2 Antibody - BSA Free [NBP1-32781] -

Molecular and biochemical characterization of GFAP-Coasy mice. (A) Relative Coasy mRNA expression at 15 days and 6 months in Ctrl (n = 4) and GFAP-Coasy (n = 4) mice brain, tested by qPCR. (B) Representative images of Western blot analysis and (C–J) densitometric quantification of (C) Coasy, (D) Dcx, (E) Gfap, (F) Ft-H, (G) Ft-L, (H) Dmt1, (I) TfR1, and (J) Aco2 at 15 days and 6 months in Ctrl and GFAP-Coasy mice brain. (K) Specific activity of mitochondrial respiratory chain complex I, expressed as nmoles/min/mg of protein. *p < 0.05, ** p < 0.01; ***p < 0.001 (two-way ANOVA). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39301217), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Aconitase 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aconitase 2

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Alternate Names

Aconitase, aconitase 2, mitochondrial, aconitate hydratase, mitochondrial, ACONM, Citrate hydro-lyase, EC 4.2.1, EC 4.2.1.3, MGC20605, MGC33908

Gene Symbol

ACO2

Additional Aconitase 2 Products

Product Documents for Aconitase 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Aconitase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Aconitase 2 Antibody - BSA Free

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Protocols

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