Aconitase 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15245

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Zebrafish

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Aconitase 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (98%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Aconitase 2 Antibody - BSA Free

Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] diluted at 1:10000.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] dilution: 1:500.
Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE; antibody diluted at 1:10000.
Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody, antibody diluted at 1:1000.
Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245]

Western Blot: Aconitase 2 Antibody [NBP2-15245] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] dilution: 1:500.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] ) dilution: 1:500.
Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245]

Immunohistochemistry-Paraffin: Aconitase 2 Antibody [NBP2-15245] - Paraffin-embedded mouse ovary. Aconitase 2 antibody [N1N3] dilution: 1:500
Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245]

Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245]

Immunoprecipitation: Aconitase 2 Antibody [NBP2-15245] - Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 ug of Aconitase 2 antibody [N1N3]. Western blot analysis was performed using Aconitase 2 antibody [N1N3].EasyBlot anti-Rabbit IgG was used as a secondary reagent
Aconitase 2 Antibody

Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] -

Immunohistochemistry: Aconitase 2 Antibody [NBP2-15245] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (NBP2-15245) at 1:300 dilution.
Aconitase 2 Antibody

Western Blot: Aconitase 2 Antibody [NBP2-15245] -

Western Blot: Aconitase 2 Antibody [NBP2-15245] - Sample (30 ug of whole cell lysate)
A: zebrafish eye
7.5% SDS PAGE
NBP2-15245 diluted at 1:1000
Aconitase 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Aconitase 2 Antibody [NBP2-15245] -

Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (NBP2-15245) diluted at 1:1000.
Red: MitoTrackerR Red CMXRos, a mitochondria tracker.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Aconitase 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Aconitase 2

The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]

Alternate Names

Aconitase, aconitase 2, mitochondrial, aconitate hydratase, mitochondrial, ACONM, Citrate hydro-lyase, EC 4.2.1, EC 4.2.1.3, MGC20605, MGC33908

Gene Symbol

ACO2

UniProt

Q99798 (Human)

Additional Aconitase 2 Products

Product Documents for Aconitase 2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Aconitase 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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