ACSL3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15252

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (96%), Porcine (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ACSL3 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ACSL3. The exact sequence is proprietary.

Localization

Mitochondrion outer membrane; Single-pass type III membrane protein; Peroxisome membrane; Microsome membrane; Endoplasmic reticulum membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACSL3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ACSL3 Antibody [NBP2-15252]

Immunocytochemistry/ Immunofluorescence: ACSL3 Antibody [NBP2-15252]

Immunocytochemistry/Immunofluorescence: ACSL3 Antibody [NBP2-15252] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ACSL3 stained by ACSL3 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Human breast carcinoma. ACSL3 stained by ACSL3 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252]

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - IHC analysis of human breast cancer tissue section, using ACSL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer for 15 min.
Simple Western: ACSL3 Antibody [NBP2-15252]

Simple Western: ACSL3 Antibody [NBP2-15252]

Simple Western: ACSL3 Antibody [NBP2-15252] - Mouse brain (hippocampus) tissue. Simple Western image submitted by a verified customer review.
ACSL3 Antibody

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] -

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] Mouse stomach. ACSL3 stained by ACSL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ACSL3 Antibody

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] -

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - Rat colon.ACSL3 stained by ACSL3 antibody diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ACSL3 Antibody

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] -

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ACSL3 Antibody

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] -

Immunohistochemistry-Paraffin: ACSL3 Antibody [NBP2-15252] - ACSL3 antibody detects ACSL3 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ACSL3 stained by ACSL3 antibody (NBP2-15252) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ACSL3 Antibody - BSA Free

Western Blot: ACSL3 Antibody [NBP2-15252] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 antibody (NBP2-15252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
ACSL3 Antibody - BSA Free

Western Blot: ACSL3 Antibody [NBP2-15252] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACSL3 antibody (NBP2-15252) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ACSL3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent.

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse brain (hippocampus) tissue, separated by Size

Reviewed Applications

Read 1 review rated 5 using NBP2-15252 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ACSL3

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

ACS3EC 6.2.1.3, acyl-CoA synthetase long-chain family member 3, FACL3lignoceroyl-CoA synthase, fatty-acid-Coenzyme A ligase, long-chain 3, LACS 3, LACS3, Long-chain acyl-CoA synthetase 3, long-chain-fatty-acid--CoA ligase 3, PRO2194

Gene Symbol

ACSL3

UniProt

O95573 (Human)

Additional ACSL3 Products

Product Documents for ACSL3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ACSL3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for ACSL3 Antibody - BSA Free

Customer Reviews for ACSL3 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • ACSL3 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Brain (hippocampus) tissue
    Species: Mouse
    Verified Customer | Posted 07/01/2020
    ACSL3 Antibody - BSA Free NBP2-15252
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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