ACTR1B Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92261

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ACTR1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human ACTR1B (NP_005726.1). MESYDIIANQPVVIDNGSGVIKAGFAGDQIPKYCFPNYVGRPKHMRVMAGALEGDLFIGPKAEEHRGLLTIRYPMEHGVVRDWNDMERIWQYVYSKDQLQTFSEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACTR1B Antibody - Azide and BSA Free

Western Blot: ACTR1B AntibodyAzide and BSA Free [NBP2-92261]

Western Blot: ACTR1B AntibodyAzide and BSA Free [NBP2-92261]

Western Blot: ACTR1B Antibody [NBP2-92261] - Western blot analysis of extracts of various cell lines, using ACTR1B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-92261] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACTR1B Rabbit pAb (NBP2-92261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-92261] - Immunohistochemistry of paraffin-embedded Mouse kidney using ACTR1B antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody - Azide and BSA Free [NBP2-92261]

Immunohistochemistry-Paraffin: ACTR1B Antibody [NBP2-92261] - Immunohistochemistry of paraffin-embedded Rat testis using ACTR1B Rabbit pAb (NBP2-92261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for ACTR1B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACTR1B

In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90% amino acid identity and 96% amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be ivolved in dynein-driven vesicle movement.

Alternate Names

Actin-related protein 1B, ARP1 (actin-related protein 1, yeast) homolog B (centractin beta), ARP1 actin-related protein 1 homolog B, centractin beta (yeast), ARP1BPC3, centractin beta, CTRN2beta-centractin

Gene Symbol

ACTR1B

Additional ACTR1B Products

Product Documents for ACTR1B Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ACTR1B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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