Acylglycerol Kinase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-49085

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all AGK Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: IVKGETVPLDVLQIKGEKEQPVFAMTGLRWGSFRDAGVKVSKYWYLGPLKIKAAHFFSTLKEWPQTHQASISYTGPTERPPNEPEETPVQRPSLYRRILRRLASYWAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Acylglycerol Kinase Antibody - BSA Free

Western Blot: Acylglycerol Kinase Antibody [NBP2-49085]

Western Blot: Acylglycerol Kinase Antibody [NBP2-49085]

Western Blot: Acylglycerol Kinase Antibody [NBP2-49085] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: Acylglycerol Kinase Antibody [NBP2-49085]

Immunocytochemistry/ Immunofluorescence: Acylglycerol Kinase Antibody [NBP2-49085]

Immunocytochemistry/Immunofluorescence: Acylglycerol Kinase Antibody [NBP2-49085] - Staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP2-49085]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP2-49085]

Immunohistochemistry-Paraffin: Acylglycerol Kinase Antibody [NBP2-49085] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for Acylglycerol Kinase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AGK

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth

Long Name

Acylglycerol Kinase

Alternate Names

FLJ10842, MULK

Gene Symbol

AGK

Additional AGK Products

Product Documents for Acylglycerol Kinase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Acylglycerol Kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Acylglycerol Kinase Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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