ADAM10 Antibody (CL13815) - BSA Free

Novus Biologicals | Catalog # NBP3-24580

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL13815

Format

BSA Free
View all ADAM10 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from O14672, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ADAM10 Antibody (CL13815) - BSA Free

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining of human fallopian tube shows strong membranous and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining of human anaplastic oligodendroglioma shows strong cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining of human prostate shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Analysis in human prostate and skeletal muscle tissues using NBP3-24580 antibody. Corresponding ADAM10 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining in RH-30 cell line with Anti-ADAM10 monoclonal antibody, showing specific staining of Golgi apparatus and nucleus in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry-Paraffin: ADAM10 Antibody (CL13815) [NBP3-24580]

Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.

Applications for ADAM10 Antibody (CL13815) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Gene Symbol

ADAM10

Additional ADAM10 Products

Product Documents for ADAM10 Antibody (CL13815) - BSA Free

Certificate of Analysis

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Product Specific Notices for ADAM10 Antibody (CL13815) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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