ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-12015

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # RM0146-7H12

Format

Azide and BSA Free
View all ADAM10 Primary Antibodies »

Product Specifications

Immunogen

Mouse recombinant ADAM10

Specificity

Detects mouse ADAM10 and cross reacts with human ADAM10. Weak cross-reactivity is observed to ADAM9 and ADAM15 in Western blotting.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Scientific Data Images for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

Immunohistochemistry-Paraffin: ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free [NBP2-12015]

Immunohistochemistry-Paraffin: ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free [NBP2-12015]

Immunohistochemistry-Paraffin: ADAM10 Antibody (RM0146-7H12) [NBP2-12015] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP2-12015

Applications for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1000

Immunofluorescence

1:500-1:1000

Western Blot

1:500-1:1000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAM10

Proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).

Long Name

A Disintegrin and Metalloprotease-like Domain 10

Alternate Names

CD156c, Kuzbanian, MADM

Gene Symbol

ADAM10

UniProt

O35598

Additional ADAM10 Products

Product Documents for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ADAM10 Antibody (RM0146-7H12) - Azide and BSA Free

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Protocols

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FAQs

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